Patent: 5,356,804
✉ Email this page to a colleague
Summary for Patent: 5,356,804
Title: | Cloning and expression of biologically active human .alpha.-galactosidase A |
Abstract: | The present invention involves the production of large quantities of human .alpha.-Gal A by cloning and expressing the .alpha.-Gal A coding sequence in eukaryotic host cell expression systems. The eukaryotic expression systems, and in particular the mammalian host cell expression system described herein provide for the appropriate cotranslational and posttranslational modifications required for proper processing, e.g., glycosylation, phosphorylation, etc. and sorting of the expression product so that an glycosylation, phosphorylation, etc. and sorting of the expression product so that an active enzyme is produced. In addition, the expression of fusion proteins which simplify purification is described. Using the methods described herein, the recombinant .alpha.-Gal A is secreted by the engineered host cells so that it is recovered from the culture medium in good yield. The .alpha.-Gal A produced in accordance with the invention may be used in the treatment in Fabry Disease; for the hydrolysis of .alpha.-galactosyl residues in glycoconjugates; and/or for the conversion of the blood group B antigen on erythrocytes to the blood group O antigen. |
Inventor(s): | Desnick; Robert J. (New York, NY), Bishop; David F. (New York, NY), Ioannou; Yiannis A. (New York, NY) |
Assignee: | Mount Sinai School of Medicine of the City of New York (New York, NY) |
Application Number: | 07/602,824 |
Patent Claims: | see list of patent claims |
Details for Patent 5,356,804
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Genzyme Corporation | FABRAZYME | agalsidase beta | For Injection | 103979 | 04/24/2003 | ⤷ Try a Trial | 2039-02-26 |
Genzyme Corporation | FABRAZYME | agalsidase beta | For Injection | 103979 | 10/10/2003 | ⤷ Try a Trial | 2039-02-26 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
International Patent Family for US Patent 5,356,804
Country | Patent Number | Estimated Expiration |
---|---|---|
World Intellectual Property Organization (WIPO) | 9412628 | ⤷ Try a Trial |
World Intellectual Property Organization (WIPO) | 9207936 | ⤷ Try a Trial |
United States of America | 5580757 | ⤷ Try a Trial |
>Country | >Patent Number | >Estimated Expiration |