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Last Updated: March 24, 2023

Patent: 5,308,763

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Summary for Patent: 5,308,763
Title: Method of making primary culture of olfactory neurons
Abstract:A method of producing primary cultures of olfactory neurons which are purified from sustentacular cells and basal cells. The neuronal cells demonstrate responsiveness to physiologic levels of odorants and express Olfactory Marker Protein (OMP). The steps required to obtain the primary cultures are: 1. providing olfactory epithelium of an animal; 2. dissociation of neuronal cells using enzymatic digestion; 3. filtering using a mesh filter having a pore size between 10 and 25 microns to separate cell aggregates; 4. removal of the cell aggregates; and 5. plating the dissociated neuronal cells in a nutrient medium containing D-valine, Nerve Growth Factor (NGF), and other significant ingredients to obtain a culture of olfactory neurons. In addition to OMP the neurons are capable of expressing vimentin, neuron-specific enolase but not expressing glial fibrillary acidic proteins, S-100 protein, keratin, or neurofilament protein.
Inventor(s): Ronnett; Gabriele V. (Baltimore, MD), Hester; Lynda (Towson, MD), Snyder; Solomon H. (Baltimore, MD)
Assignee: The Johns Hopkins University (Baltimore, MD)
Application Number:07/848,687
Patent Claims:see list of patent claims

Details for Patent 5,308,763

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Bausch & Lomb Incorporated VITRASE hyaluronidase Injection 021640 05/05/2004 See Plans and Pricing 2011-10-01
Bausch & Lomb Incorporated VITRASE hyaluronidase Injection 021640 12/02/2004 See Plans and Pricing 2011-10-01
Amphastar Pharmaceuticals, Inc. AMPHADASE hyaluronidase Injection 021665 10/26/2004 See Plans and Pricing 2011-10-01
Akorn, Inc. HYDASE hyaluronidase Injection 021716 10/25/2005 See Plans and Pricing 2011-10-01
Smith & Nephew, Inc. SANTYL collagenase Ointment 101995 06/04/1965 See Plans and Pricing 2011-10-01
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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