Patent: 5,308,763

Summary for Patent: 5,308,763

Title:	Method of making primary culture of olfactory neurons
Abstract:	A method of producing primary cultures of olfactory neurons which are purified from sustentacular cells and basal cells. The neuronal cells demonstrate responsiveness to physiologic levels of odorants and express Olfactory Marker Protein (OMP). The steps required to obtain the primary cultures are: 1. providing olfactory epithelium of an animal; 2. dissociation of neuronal cells using enzymatic digestion; 3. filtering using a mesh filter having a pore size between 10 and 25 microns to separate cell aggregates; 4. removal of the cell aggregates; and 5. plating the dissociated neuronal cells in a nutrient medium containing D-valine, Nerve Growth Factor (NGF), and other significant ingredients to obtain a culture of olfactory neurons. In addition to OMP the neurons are capable of expressing vimentin, neuron-specific enolase but not expressing glial fibrillary acidic proteins, S-100 protein, keratin, or neurofilament protein.
Inventor(s):	Ronnett; Gabriele V. (Baltimore, MD), Hester; Lynda (Towson, MD), Snyder; Solomon H. (Baltimore, MD)
Assignee:	The Johns Hopkins University (Baltimore, MD)
Application Number:	07/848,687
Patent Claims:	see list of patent claims

Details for Patent 5,308,763

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Bausch & Lomb Incorporated	VITRASE	hyaluronidase	Injection	021640	05/05/2004		2011-10-01
Bausch & Lomb Incorporated	VITRASE	hyaluronidase	Injection	021640	12/02/2004		2011-10-01
Amphastar Pharmaceuticals, Inc.	AMPHADASE	hyaluronidase	Injection	021665	10/26/2004		2011-10-01
Akorn, Inc.	HYDASE	hyaluronidase	Injection	021716	10/25/2005		2011-10-01
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2011-10-01