Patent: 5,001,048
✉ Email this page to a colleague
▶ Start for $1
Remove trial restrictions
Summary for Patent: 5,001,048
| Title: | Electrical biosensor containing a biological receptor immobilized and stabilized in a protein film |
| Abstract: | An electrical biosensor for analyte determination is prepared by polymerization of a mixture of a biological receptor capable of binding an analyte in a sample, a protein and a polymerizing agent such as glutaraldehyde to form a polymeric film on a transducer. The mixture preferably contains a stabilizer selected from lipids, detergents and antioxidents. The receptor may be an acetylcholine receptor and the analyte, acetylcholine. A preferred stabilizer is a combination of phosphatidyl choline and octyphenoxypolyethoxyethanol. In carrying out a determination, analyte in a sample binds to the receptor causing a change in an electrical characteristic of the film which is indicative of the presence of the analyte. The biosensor may contain a second polymeric film that is free of the receptor and which serves as a control. |
| Inventor(s): | Taylor; Richard F. (Boxford), Marenchic; Ingrid G. (Walpole), Cook; Edward J. (South Hamilton, MA) |
| Assignee: | Aurthur D. Little, Inc. (Cambridge, MA) |
| Application Number: | 07/058,389 |
| Patent Claims: | see list of patent claims |
