Serum-free animal cell culture medium and methods for the primary culture and production of cell lines using this medium
A serum-free animal tissue culture medium contains a mixture of six fatty acids and albumin or dextran. The medium is particularly adapted for the primary culture of rat liver epithelial cells and possibly in the presence of hormones and/or growth factors, for obtaining cell lines, in particular of myelomae and hybridomae.
Chessebeuf; Martina L. (Dijon, FR), Padieu; Prudent H. (Chevigny St Sauveur, FR)
Institut National de la Sante et de la Recherche Medicale (Paris, FR)
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