Claims for Patent: 9,994,849
✉ Email this page to a colleague
Summary for Patent: 9,994,849
Title: | Method of separating senescent cells using overexpression of protocadherin |
Abstract: | Provided is a method of separating senescent cells from a sample including the senescent cells, and a method of removing senescent cells from a sample or a subject including the senescent cells. |
Inventor(s): | Kim; Byungju (Suwon-si, KR), Ryu; Sungjin (Yongin-si, KR), Park; Sangchul (Seongnam-si, KR) |
Assignee: | SAMSUNG ELECTRONICS CO., LTD. (Suwon-si, KR) |
Application Number: | 15/387,346 |
Patent Claims: | 1. A method of separating senescent cells from a sample, the method comprising culturing a sample containing senescent cells and young cells in a culture dish without
pretreatment of the culture dish for cell culture, wherein the senescent cells adhere to the culture dish, but the young cells do not adhere to the culture dish; removing the non-adherent young cells from the sample; and separating the adherent
senescent cells from the culture dish.
2. The method of claim 1, wherein the senescent cells show an increased expression level of messenger RNA (mRNA) of protocadherin or a protocadherin protein than a young cell, an increased DNA methylation level in a protocadherin gene cluster than a young cell, .beta.-galactosidase activity, a flat morphology, or a combination thereof. 3. The method of claim 1, wherein the culture dish does not comprise a coating with gelatin, collagen, fibronectin, polylysine, vitronectin, osteopontin, hydrogel, laminin, a fragment thereof, or a mimetic thereof does not comprise modification of the surface of the culture dish with a compound having a hydroxy group or a carboxyl group; does not comprise a coating with Matrigel; does not comprise a coating with an extracellular matrix or a fragment thereof; or a combination thereof. 4. The method of claim 1, wherein the culture dish is made of plastic, glass, or a combination thereof. 5. The method of claim 4, wherein the plastic is polystyrene. 6. The method of claim 1, wherein the culturing is performed for about 30 minutes to about 24 hours. 7. The method of claim 1, wherein separating the adherent senescent cells from the culture dish comprises applying trypsin, collagenase, hyaluronidase, DNase, elastase, papain, protease type XIV, or a combination thereof to the senescent cells adhering to the culture dish. 8. The method of claim 1, further comprising culturing the separated senescent cells in a cell culture vessel. |
Details for Patent 9,994,849
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Bausch & Lomb Incorporated | VITRASE | hyaluronidase | Injection | 021640 | 05/05/2004 | ⤷ Try a Trial | 2035-12-24 |
Bausch & Lomb Incorporated | VITRASE | hyaluronidase | Injection | 021640 | 12/02/2004 | ⤷ Try a Trial | 2035-12-24 |
Amphastar Pharmaceuticals, Inc. | AMPHADASE | hyaluronidase | Injection | 021665 | 10/26/2004 | ⤷ Try a Trial | 2035-12-24 |
Akorn, Inc. | HYDASE | hyaluronidase | Injection | 021716 | 10/25/2005 | ⤷ Try a Trial | 2035-12-24 |
Smith & Nephew, Inc. | SANTYL | collagenase | Ointment | 101995 | 06/04/1965 | ⤷ Try a Trial | 2035-12-24 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
Make Better Decisions: Try a trial or see plans & pricing
Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.