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Last Updated: March 28, 2024

Claims for Patent: 9,994,849


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Summary for Patent: 9,994,849
Title:Method of separating senescent cells using overexpression of protocadherin
Abstract: Provided is a method of separating senescent cells from a sample including the senescent cells, and a method of removing senescent cells from a sample or a subject including the senescent cells.
Inventor(s): Kim; Byungju (Suwon-si, KR), Ryu; Sungjin (Yongin-si, KR), Park; Sangchul (Seongnam-si, KR)
Assignee: SAMSUNG ELECTRONICS CO., LTD. (Suwon-si, KR)
Application Number:15/387,346
Patent Claims:1. A method of separating senescent cells from a sample, the method comprising culturing a sample containing senescent cells and young cells in a culture dish without pretreatment of the culture dish for cell culture, wherein the senescent cells adhere to the culture dish, but the young cells do not adhere to the culture dish; removing the non-adherent young cells from the sample; and separating the adherent senescent cells from the culture dish.

2. The method of claim 1, wherein the senescent cells show an increased expression level of messenger RNA (mRNA) of protocadherin or a protocadherin protein than a young cell, an increased DNA methylation level in a protocadherin gene cluster than a young cell, .beta.-galactosidase activity, a flat morphology, or a combination thereof.

3. The method of claim 1, wherein the culture dish does not comprise a coating with gelatin, collagen, fibronectin, polylysine, vitronectin, osteopontin, hydrogel, laminin, a fragment thereof, or a mimetic thereof does not comprise modification of the surface of the culture dish with a compound having a hydroxy group or a carboxyl group; does not comprise a coating with Matrigel; does not comprise a coating with an extracellular matrix or a fragment thereof; or a combination thereof.

4. The method of claim 1, wherein the culture dish is made of plastic, glass, or a combination thereof.

5. The method of claim 4, wherein the plastic is polystyrene.

6. The method of claim 1, wherein the culturing is performed for about 30 minutes to about 24 hours.

7. The method of claim 1, wherein separating the adherent senescent cells from the culture dish comprises applying trypsin, collagenase, hyaluronidase, DNase, elastase, papain, protease type XIV, or a combination thereof to the senescent cells adhering to the culture dish.

8. The method of claim 1, further comprising culturing the separated senescent cells in a cell culture vessel.

Details for Patent 9,994,849

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Bausch & Lomb Incorporated VITRASE hyaluronidase Injection 021640 05/05/2004 ⤷  Try a Trial 2035-12-24
Bausch & Lomb Incorporated VITRASE hyaluronidase Injection 021640 12/02/2004 ⤷  Try a Trial 2035-12-24
Amphastar Pharmaceuticals, Inc. AMPHADASE hyaluronidase Injection 021665 10/26/2004 ⤷  Try a Trial 2035-12-24
Akorn, Inc. HYDASE hyaluronidase Injection 021716 10/25/2005 ⤷  Try a Trial 2035-12-24
Smith & Nephew, Inc. SANTYL collagenase Ointment 101995 06/04/1965 ⤷  Try a Trial 2035-12-24
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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