Claims for Patent: 9,964,541

Summary for Patent: 9,964,541

Title:	Method and devices for capturing circulating tumor
Abstract:	A method of capturing a Circulating Tumor Cell (CTC) from a sample includes introducing a sample into a microfluidic device having a cell capture surface and a flow modification surface under conditions that allow a CTC to bind to a cell rolling-inducing agent and a capturing agent disposed on the cell capture surface. The flow modification surface induces a rotational flow within the sample as it flows through the microfluidic device.
Inventor(s):	Hong; Seungpyo (Naperville, IL), Myung; Ja Hye (Daejeon, KR), Eddington; David (Wheaton, IL), Launiere; Cari (Chicago, IL)
Assignee:	THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS (Urbana, IL)
Application Number:	15/016,005
Patent Claims:	1. A method of capturing a Circulating Tumor Cell (CTC) from a sample comprising the step of introducing said sample into a microfluidic device under conditions that allow a CTC to bind to a cell rolling-inducing agent and a capturing agent, the device comprising (i) an immobilized cell rolling-inducing agent, and (ii) an immobilized capturing agent, wherein the capturing agent is an antibody that specifically binds to a CTC and is immobilized via attachment to a modified poly(amidoamine) dendrimer covalently attached to the surface of the device and wherein the dendrimer organizes multiple capturing agents on the surface of the device to facilitate a multivalent effect. 2. The method of claim 1 further comprising applying a shear stress between 0.05 and 10 dyn/cm.sup.2 on the sample introduced into the device. 3. The method of claim 2 wherein the shear stress is between 0.1 and 2.0 dyn/cm.sup.2. 4. The method of claim 3 wherein the shear stress is about 0.16 dyn/cm.sup.2. 5. The method of claim 1 wherein the cell rolling-inducing agent is a selectin or a CTC binding fragment of a selectin. 6. The method of claim 5 wherein the selectin is selected from the group consisting of E-selectin, P-selectin, and L-selectin. 7. The method of claim 1 wherein the antibody is one or more of anti-EpCAM, trastuzumab, bevacizumab, anti-CD33 antibody, anti-CD20 antibody and fragments thereof. 8. The method of claim 1 wherein the modified poly(amidoamine) dendrimer is selected from the group consisting of a generation 3, a generation 4, a generation 5, a generation 6, a generation 7, a generations 8, and a generation 9 modified poly(amidoamine) dendrimer. 9. The method of claim 1 wherein a linear polymer is attached to the surface of the device and the dendrimer is attached the linear polymer on the surface of the device. 10. The method of claim 9 wherein the linear polymer is poly(ethylene glycol). 11. The method of claim 1, wherein the immobilized cell rolling-inducing agent and the immobilized capturing agent are arranged in a substantially uniform manner. 12. The method of claim 1, wherein the immobilized cell rolling-inducing agent and the immobilized capturing agent are arranged in a pattern. 13. A microfluidic device for capturing a Circulating Tumor Cell (CTC) from a sample, comprising: a channel comprising a cell capture surface and a flow modification surface, wherein the cell capture surface comprises a cell rolling-inducing agent and a capturing agent immobilized on the cell capture surface, wherein the capturing agent is an antibody that specifically binds to a CTC and is immobilized via attachment to a modified poly(amidoamine) dendrimer covalently attached to the surface of the device and wherein the dendrimer organizes multiple capturing agents on the surface of the device to facilitate a multivalent effect. 14. The device of claim 13, wherein the cell rolling-inducing agent is a selectin or a CTC binding fragment of a selectin. 15. The device of claim 13, wherein the antibody is one or more of anti-EpCAM, trastuzumab, bevacizumab, anti-CD33 antibody, anti-CD20 antibody and fragments thereof. 16. The device of claim 13 wherein the modified poly(amidoamine) dendrimer is selected from the group consisting of a generation 3, a generation 4, a generation 5, a generation 6, a generation 7, a generations 8, and a generation 9 modified poly(amidoamine) dendrimer. 17. The device of claim 13 wherein a linear polymer is attached to the surface of the device and the dendrimer is attached to the linear polymer on the surface of the device. 18. The device of claim 17 wherein the linear polymer is poly(ethylene glycol). 19. The device of claim 13, wherein the immobilized cell rolling-inducing agent and the immobilized capturing agent are arranged in a substantially uniform manner. 20. The device of claim 13, wherein the immobilized cell rolling-inducing agent and the immobilized capturing agent are arranged in a pattern.

Details for Patent 9,964,541

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Genentech, Inc.	HERCEPTIN	trastuzumab	For Injection	103792	09/25/1998		2029-04-25
Genentech, Inc.	HERCEPTIN	trastuzumab	For Injection	103792	02/10/2017		2029-04-25
Genentech, Inc.	AVASTIN	bevacizumab	Injection	125085	02/26/2004		2029-04-25