You’re using a public version of DrugPatentWatch with 5 free searches available | Register to unlock more free searches. CREATE FREE ACCOUNT

Last Updated: March 28, 2024

Claims for Patent: 9,902,937


✉ Email this page to a colleague

« Back to Dashboard


Summary for Patent: 9,902,937
Title:Methods for culturing minimally-passaged fibroblasts and uses thereof
Abstract: The present invention relates to novel methods of growing at least 100 million minimally-passaged fibroblasts from a small biopsy specimen. The invention includes methods wherein a small biopsy specimen is seeded directly into a large tissue culture flask, and passaged no more than three times.
Inventor(s): Maslowski; John (Pottstown, PA), Thomas; Myrna F. (Hainesport, NJ), Lindner; Marie A. (Radnor, PA)
Assignee: Fibrocell Technologies, Inc. (Exton, PA)
Application Number:14/280,957
Patent Claims:1. A topical composition comprising fibroblast conditioned medium, wherein the topical composition is produced by the method comprising the steps of: digesting a dermal biopsy specimen obtained from a human and comprising fibroblasts with a dissociative or digestive enzyme to form a fibroblast cell suspension, wherein the dermal biopsy specimen is from 1 to 5 mm in thickness, and between 4 and 12 cm.sup.2 in area; seeding the fibroblast cell suspension into a first tissue culture flask; culturing the fibroblast cell suspension in the first tissue culture flask through at most three passages into a sequentially larger tissue culture flask; harvesting the fibroblast conditioned medium from the fibroblast cell suspension during the culturing step, wherein the fibroblast cell suspension comprises 3.times.10.sup.8 or more cells at harvest; and formulating the conditioned medium into the topical composition.

2. The composition of claim 1, wherein the fibroblast cell suspension is passaged twice.

3. The composition of claim 1, wherein the fibroblast cell suspension is passaged once.

4. The composition of claim 1, wherein the enzyme is a collagenase.

5. The composition of claim 4, wherein the collagenase is liberase.

6. The composition of claim 1, wherein the enzyme is trypsin.

7. The composition of claim 1, wherein the first tissue culture flask is a T-125 flask, T-150 flask, T-175 flask, or T-225 flask.

8. The composition of claim 1, wherein the sequentially larger tissue culture flask is a T-500 flask or multi-layer culture stack.

Make Better Decisions: Try a trial or see plans & pricing

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.