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Generated: August 23, 2019

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Claims for Patent: 9,902,933

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Summary for Patent: 9,902,933
Title:Method of producing retinal pigment epithelial cell sheet
Abstract: The present invention provides a method of producing a cell sheet including the following steps (1) seeding and culturing retinal pigment epithelial cells on a collagen gel to form a cell sheet composed of the retinal pigment epithelial cells, and (2) degrading the collagen gel with collagenase to detach the cell sheet composed of the retinal pigment epithelial cells, and the like.
Inventor(s): Takahashi; Masayo (Wako, JP), Okamoto; Satoshi (Wako, JP), Kamao; Hiroyuki (Wako, JP)
Assignee: RIKEN (Wako, JP)
Application Number:14/001,108
Patent Claims:1. A method of producing a cell sheet comprising the following steps (a) seeding and culturing retinal pigment epithelial cells on a swine tendon-derived fibrotic collagen gel to form a cell sheet composed of the retinal pigment epithelial cells, and (b) degrading the swine tendon-derived fibrotic collagen gel with collagenase to detach the cell sheet composed of the retinal pigment epithelial cells wherein the concentration of collagen in the swine tendon-derived fibrotic collagen gel is 0.21-0.35%.

2. The production method of the cell sheet according to claim 1, wherein the retinal pigment epithelial cells are cells obtained by inducing differentiation of stem cells or progenitor cells.

3. The production method of the cell sheet according to claim 2, wherein the stem cells are embryonic stem (ES) cells or induced pluripotent stem (iPS) cells.

4. The production method of the cell sheet according to claim 1, further comprising the following step (c): (c) confirming the presence or absence of a basement membrane on the contact surface between the detached cell sheet and the swine tendon-derived fibrotic collagen gel.

5. The production method of the cell sheet according to claim 2, further comprising the following step (c): (c) confirming the presence or absence of a basement membrane on the contact surface between the detached cell sheet and the swine tendon-derived fibrotic collagen gel.

6. The production method of the cell sheet according to claim 3, further comprising the following step (c): (c) confirming the presence or absence of a basement membrane on the contact surface between the detached cell sheet and the swine tendon-derived fibrotic collagen gel.

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Foriegn Application Priority Data
Foreign Country Foreign Patent Number Foreign Patent Date
Japan2011-040130Feb 25, 2011
PCT Information
PCT FiledFebruary 24, 2012PCT Application Number:PCT/JP2012/054631
PCT Publication Date:August 30, 2012PCT Publication Number:WO2012/115244

Details for Patent 9,902,933

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Advance Biofactures SANTYL collagenase VIAL 101995 001 1965-06-04   Try a Free Trial RIKEN (Wako, JP) 2031-02-25 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

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