Claims for Patent: 9,828,595
✉ Email this page to a colleague
Summary for Patent: 9,828,595
Title: | Thermostable asparaginase variants and polynucleotides encoding same |
Abstract: | The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism. |
Inventor(s): | Matsui; Tomoko (Chiba, JP), Tomiki; Aki (Chiba, JP), Svendsen; Allan (Hoersholm, DK), Hendriksen; Hanne Vang (Holte, DK), Stringer; Mary Ann (Soeborg, DK), Ayabe; Keiichi (Chiba, JP) |
Assignee: | Novozymes A/S (Bagsvaerd, DK) |
Application Number: | 14/420,399 |
Patent Claims: | 1. An asparaginase variant comprising a substitution at one or more positions corresponding to positions 140 or 241 of SEQ ID NO: 2, or a deletion at a position
corresponding to position 27 of SEQ ID NO: 2, wherein the variant has at least 80% sequence identity to the mature polypeptide of either of SEQ ID NOs: 2 or 4, and wherein the variant has asparaginase activity.
2. The variant of claim 1, in which the amino acid at a position corresponding to position 140 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Trp, Tyr, or Val. 3. The variant of claim 1, in which the amino acid at a position corresponding to position 140 is substituted with Asp. 4. The variant of claim 1, in which the amino acid at a position corresponding to position 241 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Ser, Thr, Trp, Tyr, or Val. 5. The variant of claim 1, in which the amino acid at a position corresponding to position 241 is substituted with Glu. 6. The variant of claim 1, which further comprises one or more of the following substitutions: K122A, K122R, D197E, S238C, N239C, K240R, K253R, I258V, I258Y, R259C, R259V, S297V or E373H. 7. The variant of claim 1, which further comprises a substitution at one or more positions corresponding to positions 71, 74, 139, 194, 228, 299, 314, 333, 334, 337, 338, 356 or 363 of SEQ ID NO: 2. 8. The variant of claim 1, which further comprises one or more of the following substitutions: T71C, T74A, T74C, V139G, K194L, I228M, S299A, T314A, P333L, S334P, S334W, E337S, S338G, S338W, G356D or K363R. 9. The variant of claim 1, which further comprises a substitution at one or more positions corresponding to positions 70, 307, 323, 327, 349, 351 or 353 of SEQ ID NO: 2. 10. The variant of claim 1, which further comprises one or more of the following substitutions: N70K, S307A, A323R, T327V, A349Q, S351A or V353I. 11. A method for producing a food product from a food material, comprising: a) adding the variant of claim 1 to the food material; and b) heating the enzyme treated food material. 12. The variant of claim 1, which has at least 85% sequence identity to the mature polypeptide of any of SEQ ID NOs: 2 or 4. 13. The variant of claim 1, which has at least 90% sequence identity to the mature polypeptide of any of SEQ ID NOs: 2 or 4. 14. The variant of claim 1, which has at least 95% sequence identity to the mature polypeptide of any of SEQ ID NOs: 2 or 4. |
Details for Patent 9,828,595
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Recordati Rare Diseases, Inc. | ELSPAR | asparaginase | For Injection | 101063 | 01/10/1978 | ⤷ Try a Trial | 2032-08-17 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
Make Better Decisions: Try a trial or see plans & pricing
Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.