Claims for Patent: 9,723,854

Summary for Patent: 9,723,854

Title:	Heat-stable asparaginase for reducing acrylamide in food stuffs or stimulants
Abstract:	The invention relates to the use of an amidohydrolase for preparing foodstuffs or stimulants.
Inventor(s):	Greiner-Stoeffele; Thomas (Leipzig, DE), Struhalla; Marc (Leipzig, DE)
Assignee:	C-LECTA GMBH (Leipzig, DE)
Application Number:	12/663,800
Patent Claims:	1. A method of preparing a foodstuff or a stimulant, said method comprising: treating the foodstuff, stimulant or a precursor thereof with an amidohydrolase, the amidohydrolase having an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 2, and the amidohydrolase after an incubation duration of 5 min at 50.degree. C. having a residual activity of at least 75%. 2. The method according to claim 1, wherein the amidohydrolase has a temperature optimum in the range of 70.degree. to 120.degree. C. 3. The method according to claim 1, wherein the amidohydrolase has a specific activity of at least 200 units/mg at a temperature in the range of 60.degree. to 120.degree. C. 4. The method according to claim 1, wherein the amidohydrolase has a pH optimum of pH 1 to pH 14. 5. The method according to claim 1, wherein the amidohydrolase after storage at 4.degree. C. over a period of 1 month has a residual activity of at least 80%. 6. The method according to claim 1, wherein over the pH range of pH 5-pH 10 the amidohydrolase has an activity of at least 10% compared to its maximum activity. 7. The method according to claim 1, wherein the amidohydrolase is an asparaginase. 8. The method according to claim 7, wherein the asparaginase is "asparaginase I" from Pyrococcus furiosus. 9. The method according to claim 7, wherein the asparaginase is coded by a nucleotide sequence, which has at least 90% identity to the nucleotide sequence SEQ ID NO: 1. 10. The method according to claim 7, wherein the asparaginase is coded by the nucleotide sequence SEQ ID NO: 1. 11. The method according to claim 7, wherein the asparaginase comprises the amino acid sequence SEQ ID NO: 2. 12. The method according to claim 1, wherein preparing said foodstuff or said stimulant serves to hydrolyse asparagine to asparaginic acid. 13. The method according to claim 1, wherein preparing said foodstuff or said stimulant serves to reduce a content of asparagine and/or acrylamide in the foodstuff or stimulant. 14. The method according to claim 13, wherein the reduction in the content of asparagine occurs so that the foodstuff or stimulant has a reduced content of acrylamide during a thermal aftertreatment. 15. The method according to claim 1, wherein the foodstuff or stimulant is selected from the group consisting of crispbread, rusks, biscuits, pretzels, white toasting bread, waffles, muffins, bagels, croissants, brownies, breakfast cereals, biscotti, potato crisps, tortilla chips, corn chips, crackers, chips, rice cakes, polenta, couscous, pancakes, nuts, ready-mixed cake mixes, biscuit mixes, bread mixes, croutons, dog food, cat food, coffee beans and cocoa beans. 16. The method according to claim 15, wherein said preparing comprises decaffenating, washing or decaffenating and washing of coffee beans. 17. A process for preparing a foodstuff or a stimulant comprising the steps: (i) incubating the foodstuff or stimulant with an amidohydrolase at an incubation temperature of at least 50.degree. C., the amidohydrolase having an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 2, the amidohydrolase after an incubation duration of 5 min at 50.degree. C. having a residual activity of at least 75%; and (ii) optionally, heating the foodstuff or stimulant to a temperature lying at least 10.degree. C. above the incubation temperature. 18. The process according to claim 17, which further comprises the steps: (iii) separating the amidohydrolase from the foodstuff or stimulant or inactivating the amidohydrolase; and (iv) optionally, reusing the amidohydrolase separated in step (iii) in step (ii).

Details for Patent 9,723,854

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2027-06-13