Claims for Patent: 9,650,611

Summary for Patent: 9,650,611

Title:	Mammary artery derived cells and methods of use in tissue repair and regeneration
Abstract:	An isolated mammalian internal mammary artery-derived cell is disclosed. Furthermore, methods of isolating the mammalian internal mammary artery-derived cell are disclosed. The cell is useful in tissue engineering technologies, specifically in vascular tissue engineering.
Inventor(s):	Colter; David C. (Hamilton, NJ), Buensuceso; Charito (North Brunswick, NJ), Kazanecki; Christian C. (Martins Creek, PA), Gosiewska; Anna (Skillman, NJ)
Assignee:	DEPUY SYNTHES PRODUCTS, LLC (Raynham, MA)
Application Number:	13/671,720
Patent Claims:	1. An in vitro cell culture comprising: A culture vessel; A culture medium comprising internal mammary artery-derived cell growth medium; and A homogenous population of cells capable of self-renewal and expansion in culture derived from a mammalian internal mammary artery, Wherein said cells are positive for the expression of cell-surface marker HLA-1 and negative for expression of each one of cell-surface markers CD 10, CD31, CD34, CD45, CD133, CD141 and KDR/Flk-1, and Wherein said cells are plated in the culture vessel with the culture medium. 2. The cell culture of claim 1, wherein the cells are capable of differentiation into adipocytes, cardiomyocytes, and endothelial cells. 3. The cell culture of claim 1 wherein the culture vessel is coated. 4. The cell culture of claim 3 wherein the culture vessel is coated with collagen. 5. The cell culture of claim 1 wherein the cells are additionally positive for expression of CD29, CD44, CD73, CD166, and additionally negative for CD15, CD23, CD24, CD62p, CD80, CD86, CD104, CD117, CD138, CD146, VE-Cadherin, and HLA-2. 6. An in vitro homogenous cell culture produced by a method comprising the steps of: providing a mammalian internal mammary artery, providing an enzyme mixture wherein the enzyme mixture is comprised of digestion enzymes selected from the group consisting of a metalloprotease enzyme, a neutral protease enzyme, a mucolytic enzyme, and combinations thereof, incubating the entire mammalian internal mammary artery in the enzyme mixture for a time sufficient to remove the intimal layer and to remove external debris from the artery and provide a partially digested artery, transferring the partially digested artery into a fresh enzyme mixture and continue digestion for a time sufficient to provide a digested material, isolating and culturing the cells from the digested material to obtain a homogenous population of cells, wherein the cells are positive for the expression of cell-surface marker HLA-1 and negative for expression of CD34, suspending the cells in a culture medium, and plating the cells in a culture vessel. 7. The cell culture of claim 6 wherein the enzyme mixture is comprised of collagenase and dispase. 8. The cell culture of claim 6 wherein the time to remove the intimal layer and to remove external debris from the artery is about 60 minutes. 9. The cell culture of claim 6 wherein the time sufficient to provide digested material is in the range of about 30 minutes to about 60 minutes. 10. The cell culture of claim 6 wherein the time sufficient to saturate the enzyme mixture with digested material is about 60 minutes. 11. An implant comprising a population of isolated internal mammary artery-derived cells in a biocompatible matrix, wherein the population of isolated internal mammary artery-derived cells are capable of self-renewal and expansion in culture and are positive for the expression of cell-surface marker HLA-1 and negative for expression of each one of cell-surface markers CD 10, CD31, CD34, CD45, CD133, CD141 and KDR/F1k-1. 12. The implant of claim 11 wherein the population of isolated internal mammary artery-derived cells are additionally positive for expression of CD29, CD44, CD73, CD166, and additionally negative for CD15, CD23, CD24, CD62p, CD80, CD86, CD104, CD117, CD138, CD146, VE-Cadherin, and HLA-2. 13. The implant of claim 11 wherein the population of isolated internal mammary artery-derived cells are capable of differentiation into adipocytes, cardiomyocytes, and endothelial cells.

Details for Patent 9,650,611

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2030-09-20