Claims for Patent: 9,447,393

Summary for Patent: 9,447,393

Title:	Phosphatidic acid phosphatase gene
Abstract:	The present invention provides a novel phosphatidic acid phosphatase gene. The object of the present invention can be solved by providing a nucleic acid comprising the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 4, or SEQ ID NO: 5; a protein comprising the amino acid sequence set forth in SEQ ID NO: 2; and mutants thereof.
Inventor(s):	Ochiai; Misa (Osaka, JP)
Assignee:	SUNTORY HOLDINGS LIMITED (Osaka, JP)
Application Number:	14/235,146
Patent Claims:	1. A nucleic acid comprising: (a) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 1 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; (b) an intron-free nucleotide sequence encoding a protein that consists of an amino acid sequence having an identity of 95% or more with the amino acid sequence set forth in SEQ ID NO: 2 and that has the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; (c) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 3 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; or (d) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 5 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid. 2. A cDNA comprising a nucleic acid according to any one of (a)-(e) below: (a) a nucleotide sequence encoding a protein that consists of an amino acid sequence with deletion, substitution, or addition of 1 to 15 amino acids in the amino acid sequence set forth in SEQ ID NO: 2 and that has the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; (b) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 1 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; (c) a nucleotide sequence encoding a protein that consists of an amino acid sequence having an identity of 95% or more with the amino acid sequence set forth in SEQ ID NO: 2 and that has the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; (d) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 3 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid; or (e) a nucleotide sequence that has an identity of 95% or more with the nucleotide sequence set forth in SEQ ID NO: 5 and that encodes a protein having the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid. 3. The nucleic acid according to claim 1, wherein the nucleic acid comprises: (a) the nucleotide sequence set forth in SEQ ID NO: 1; (b) the nucleotide sequence set forth in SEQ ID NO: 3; or (c) the nucleotide sequence set forth in SEQ ID NO: 5. 4. The nucleic acid according to claim 1, wherein the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid has a higher substrate specificity for a C.sub.18 acyl group-containing phosphatidic acid than for a C.sub.17 acyl group-containing one. 5. The cDNA according to claim 2, wherein the ability to catalyze the reaction of generating diacylglycerol by dephosphorylation of phosphatidic acid has a higher substrate specificity for a C.sub.18 acyl group-containing phosphatidic acid than for a C.sub.17 acyl group-containing one. 6. A recombinant vector comprising the nucleic acid according to claim 1. 7. A transformant transformed with the recombinant vector according to claim 6, wherein the transformant is a host cell selected from a bacterial cell, a fungal cell, an isolated plant cell, or an isolated animal cell. 8. A recombinant vector comprising the cDNA according to claim 2. 9. A transformant transformed with the recombinant vector according to claim 8, wherein the transformant is a host cell selected from a bacterial cell, a fungal cell, an isolated plant cell, or an isolated animal cell.

Details for Patent 9,447,393

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2031-07-29
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2031-07-29
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2031-07-29