Claims for Patent: 9,365,865

Summary for Patent: 9,365,865

Title:	Vector utilizing borna disease virus and use thereof
Abstract:	Disclosed is a viral vector comprising (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, and having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c).
Inventor(s):	Tomonaga; Keizo (Osaka, JP), Daito; Takuji (Osaka, JP), Honda; Tomoyuki (Tokyo, JP)
Assignee:	KYOTO UNIVERSITY (Kyoto, JP)
Application Number:	13/258,698
Patent Claims:	1. A viral vector comprising: (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, containing restriction enzyme sites both at the 3' end and at the 5' end of the foreign gene, containing the sequence shown in SEQ ID NO: 11 (1) both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene and in the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the L gene, or (2) both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene and the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the M gene when the M gene of the Borna disease viral genome is contained in the same order as that in the Borna disease viral genome, and containing at least a base sequence cc inserted in the region between the restriction enzyme site at the 3' end of the foreign gene and the sequence shown in SEQ ID NO: 11, and at least a base sequence cca in the region between the restriction enzyme site at the 5' end of the foreign gene and the sequence shown in SEQ ID NO: 11, (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c), wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene and the M gene of the Borna disease virus genome are destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, and wherein an intron of the L gene of the Borna disease virus genome is deleted from (a) the cDNA of a recombinant viral RNA. 2. The viral vector according to claim 1, wherein the restriction enzyme site at the 3' end of the foreign gene is a Bst BI site, and the restriction enzyme site at the 5' end of the foreign gene is a Pac I site. 3. The viral vector according to claim 1, wherein (c) the promoter sequence is an RNA polymerase II promoter sequence. 4. A kit comprising the viral vector according to claim 1. 5. The viral vector according to claim 1, wherein the foreign gene expresses a functional RNA.

Details for Patent 9,365,865

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2029-03-31
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2029-03-31
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2029-03-31