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Last Updated: March 28, 2024

Claims for Patent: 9,206,248


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Summary for Patent: 9,206,248
Title:Vectors, host cells, and methods of production and uses
Abstract: Antibody expression vectors and plasmids can incorporate various antibody gene portions for transcription of the antibody DNA and expression of the antibody in an appropriate host cell. The expression vectors and plasmids have restriction enzyme sites that facilitate ligation of antibody-encoding DNA into the vectors. The vectors incorporate enhancer and promoter sequences that can be varied to interact with transcription factors in the host cell and thereby control transcription of the antibody-encoding DNA. A kit can incorporate these vectors and plasmids.
Inventor(s): Carton; Jill (Collegeville, PA), Lu; Jin (Boothwyn, PA), Scallon; Bernard (Wayne, PA), Snyder; Linda (Pottstown, PA)
Assignee: Janssen Biotech, Inc. (Horsham, PA)
Application Number:14/617,003
Patent Claims:1. A mammalian expression vector comprising: mammalian expression control polynucleotide sequences; and restriction sites downstream from the expression control sequences, wherein the vector comprises the nucleotide sequence of SEQ ID NO: 3.

2. The vector of claim 1, wherein the promoter sequence comprises nucleotides 35-2034 of SEQ ID NO: 3.

3. The vector of claim 2, wherein the promoter sequence is selected based on compatibility with transcription factors from a host cell.

4. The vector of claim 1 further comprising a polynucleotide sequence encoding a variable region of an antibody.

5. The vector of claim 4 wherein the polynucleotide sequence encoding a variable region encodes a light chain of an antibody.

6. The vector of claim 1, further comprising any one of the group consisting of a 5' flanking sequence, an immunoglobulin promoter region, a cloning site, an intron enhancer region, a selectable marker, and an antibiotic resistance gene.

7. The vector of claim 6 wherein expression of the selectable marker confers resistance to at least one selected from mycophenolic acid (gpt gene), hygromycin, neomycin, Zeocin.TM., kanamycin, blasticidin, and G-418.

8. The vector of claim 7 wherein the antibiotic is selected from ampicillin, chloramphenicol, and kanamycin.

9. The vector of claim 1 further comprising a secretion signal polynucleotide sequence.

10. A host cell comprising the expression vector according to claim 1.

11. The host cell of claim 10, wherein the host cell is a mammalian host cell.

12. The host cell of claim 11 wherein the host cell is a murine myeloma host cell.

13. A kit comprising the vector of claim 12.

Details for Patent 9,206,248

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2039-02-26
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2039-02-26
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2039-02-26
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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