Claims for Patent: 9,006,148

Summary for Patent: 9,006,148

Title:	Methods using a progressive cavity pump bioreactor
Abstract:	Methods of using a progressive cavity pump as a bioreactor are disclosed. Methods of isolating a biological product, such as pancreatic islet cells, using the bioreactor are also disclosed.
Inventor(s):	Zar; Harvey (Hamden, CT)
Assignee:
Application Number:	13/614,606
Patent Claims:	1. A method of preparing a biological product in a bioreactor vessel consisting of a progressive cavity pump, comprising culturing cells in a bioreactor vessel consisting of a progressive cavity pump; and isolating a biological product from the cell culture. 2. The method of claim 1, wherein the cells are eukaryotic cells or prokaryotic cells. 3. The method of claim 1, wherein the cells are cultured while being pumped through the progressive cavity pump in a culture medium at a controlled temperature and at a controlled rate. 4. The method of claim 1, wherein the biological product is a tissue explant, a protein, a nucleic acid, the cultured cells, or a fermentation product. 5. A method of isolating islet cells, comprising processing pancreatic tissue in a bioreactor vessel consisting of a progressive cavity pump to release islet cells; and collecting islet cells released from the tissue. 6. The method of claim 5, wherein the pancreatic tissue is human or porcine. 7. The method of claim 5, wherein the pancreatic tissue is pumped through the progressive cavity pump in a solution at a controlled temperature and at a controlled rate. 8. The method of claim 5, wherein processing pancreatic tissue in a bioreactor vessel consisting of a progressive cavity pump comprises contacting the pancreatic tissue in the bioreactor vessel with a glucose solution for a predetermined time, wherein the glucose solution has a concentration of glucose such that the solution has an osmotic strength at least twice that of the solution without glucose; and then contacting the pancreatic tissue in the bioreactor vessel with a physiological solution. 9. The method of claim 8, wherein the glucose solution has 600 mM glucose. 10. The method of claim 8, wherein the physiological solution is a culture medium or a physiological buffer. 11. The method of claim 8, wherein the bioreactor vessel is maintained at about 2 C to about 25 C. 12. The method of claim 5, wherein processing pancreatic tissue in a bioreactor vessel consisting of a progressive cavity pump comprises contacting the pancreatic tissue in the bioreactor vessel with a dissociation enzyme. 13. The method of claim 12, wherein the dissociation enzyme comprises a collagenase. 14. The method of claim 12, further comprising contacting the pancreatic tissue in the bioreactor vessel with a DNAse. 15. The method of claim 12, wherein the bioreactor vessel is maintained at about 37 C. 16. The method of claim 9, further comprising adding 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride to the bioreactor vessel or to pumped material exiting from the bioreactor vessel.

Details for Patent 9,006,148

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2039-03-29