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Last Updated: March 29, 2024

Claims for Patent: 8,999,684


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Summary for Patent: 8,999,684
Title:Candida tropicalis cells and use thereof
Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of .omega.-hydroxycarboxylic acids and .omega.-hydroxycarboxylic acid esters.
Inventor(s): Poetter; Markus (Muenster, DE), Hennemann; Hans-Georg (Bedburg, DE), Schaffer; Steffen (Herten, DE), Haas; Thomas (Muenster, DE)
Assignee: Evonik Degussa GmbH (Essen, DE)
Application Number:13/764,996
Patent Claims:1. A method for production of .omega.-aminocarboxylic acids or .omega.-aminocarboxylic acid esters, comprising a) contacting a Candida tropicalis cell with a medium comprising a carboxylic acid or a carboxylic acid ester, b) cultivating the Candida tropicalis cell under conditions that enable the Candida tropicalis cell to form corresponding .omega.-hydroxycarboxylic acid or .omega.-hydroxycarboxylic acid esters from the carboxylic acid or the carboxylic acid ester, c) optionally isolating the .omega.-hydroxycarboxylic acid or .omega.-hydroxycarboxylic acid esters that formed, converting the .omega.-hydroxycarboxylic acids or corresponding esters into the .omega.-oxo-carboxylic acids or the corresponding esters and then aminating the oxo-group, wherein the Candida tropicalis cell has, compared with its wild type, a reduced activity of at least one of enzyme encoded by an intron-free nucleic acid sequences selected from the group consisting of SEQ ID NO: 57 and a sequence that is 95% identical to SEQ ID NO: 57.

2. The method according to claim 1, wherein the reduced enzymatic activity comprises insertion of a foreign DNA into SEQ ID NO:57 contained in a chromosome of the Candida tropicalis cell, deletion of at least a part of SEQ ID NO:57 in a chromosome of the Candida tropicalis cell, a point mutation in SEQ ID NO:57 in a chromosome of the Candida tropicalis cell, providing an interfering RNA molecule targeting expression of SEQ ID NO:57, and exchanging a part of SEQ ID NO:57 in a chromosome of the Candida tropicalis cell with foreign DNA, and exchanging a part of a transcription promoter controlling expression of SEQ ID NO:57 in the Candida tropicalis cell with foreign DNA.

3. The method according to claim 2, wherein the foreign DNA is a selection marker gene.

4. The method according to claim 1, wherein the Candida tropicalis cell is blocked at least partially in its .beta.-oxidation.

5. The method according to claim 1, wherein the Candida tropicalis cell is obtained from a strain selected from the group consisting of Candida tropicalis H41, Candida tropicalis H41B, Candida tropicalis H51, Candida tropicalis H45, Candida tropicalis H43, Candida tropicalis H53, Candida tropicalis H534, Candida tropicalis 534B, Candida tropicalis H435, Candida tropicalis ATCC20962 and Candida tropicalis HDC100.

6. The method according to claim 1, wherein the Candida tropicalis cell is obtained from a strain selected from the group consisting of Candida tropicalis ATCC20962 and Candida tropicalis HDC100.

7. The method according to claim 1, wherein the .omega.-hydroxycarboxylic acid has a carboxylic acid chain length of from 6 to 24 or the .omega.-hydroxycarboxylic acid ester has a carboxylic acid chain length of from 6 to 24 and an alcohol chain length from 1 to 4.

8. The method according to claim 1, wherein the .omega.-hydroxycarboxylic acid or .omega.-hydroxycarboxylic acid ester is 12-hydroxydodecanoic acid or 12-hydroxydodecanoic acid methyl ester.

9. The method according to claim 1, wherein the enzyme is encoded by SEQ ID NO: 57.

10. The method according to claim 1, wherein the enzyme is encoded by a sequence that is 95% identical to SEQ ID NO: 57.

Details for Patent 8,999,684

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2029-11-11
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2029-11-11
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2029-11-11
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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