You’re using a public version of DrugPatentWatch with 5 free searches available | Register to unlock more free searches. CREATE FREE ACCOUNT

Last Updated: March 28, 2024

Claims for Patent: 8,853,380


✉ Email this page to a colleague

« Back to Dashboard


Summary for Patent: 8,853,380
Title:Modified SAK gene for the production of recombinant proteins
Abstract: The present invention relates to modified SAK gene having amino acid SEQ ID 2. The present invention further relates to process for cloning and expressing modified SAK gene fusion protein which imparts improved stability to the heterologous protein of interest. Further the invention relates to process of purification of recombinant heterologous proteins from bacterial inclusion bodies using modified SAK.
Inventor(s): Salunkhe; Shardul (Maharashtra, IN), Prasad; Bhaskarjyoti (Maharashtra, IN), Muneshwar; Praveen (Maharashtra, IN), Deshpande; Anjali Apte (Maharashtra, IN), Padmanabhan; Sriram (Maharashtra, IN)
Assignee: Lupin Limited (Mumbai, Maharashtra, IN)
Application Number:13/702,028
Patent Claims:1. A fusion DNA comprising a first DNA encoding a modified staphylokinase (SAK) protein comprising the nucleic acid sequence of SEQ ID NO. 1 and a second DNA fused in frame encoding the heterologous protein of interest.

2. The fusion DNA as claimed in claim 1, wherein the second DNA encodes the amino acid sequence as set forth in SEQ ID NO. 2.

3. The fusion DNA as claimed in claim 1, wherein the heterologous protein of interest is selected from the group comprising parathyroid hormone (1-34) as set forth in SEQ ID NO. 4, parathyroid hormone (1-84), reteplase, interferon, IL-2, IL-3, IL-4, IL-5, IL-6, IL-11 as set forth in SEQ ID NO. 6, granulocyte colony stimulating factor (GCSF), epidermal growth factor and platelet derived growth factor.

4. The fusion DNA as claimed in claim 1, wherein the heterologous protein of interest is parathyroid hormone (1-34) encoded by the nucleic acid of SEQ ID NO. 3.

5. The fusion DNA as claimed in claim 1, wherein the heterologous protein of interest is parathyroid hormone (1-34) as set forth in SEQ ID NO. 4.

6. The fusion DNA as claimed in claim 1, wherein the heterologous protein of interest is IL-11 encoded by the nucleic acid of SEQ ID NO. 5.

7. The fusion DNA as claimed in claim 1, wherein the heterologous protein of interest is IL-11 as set forth in SEQ ID NO. 6.

Make Better Decisions: Try a trial or see plans & pricing

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.