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Last Updated: March 29, 2024

Claims for Patent: 8,524,283


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Summary for Patent: 8,524,283
Title:T cell immunomodulation by placenta cell preparations
Abstract: A Method for obtaining amniotic mesenchymal tissue cells (AMTC) and/or chorionic mesenchymal tissue cells (CMTC) comprises a) isolating amniotic membrane and/or chorionic membrane from human placenta and/or separating amniotic and chorionic membrane, a) washing the membrane of step a) to remove contaminants b) cutting the membrane of step b) c) incubating the membrane fragments of step c) in a medium containing dispase for 5 to 15 minutes at 33 to 42.degree. C. d) incubating the composition of step d) in a resting solution for 5 to 15 minute at room temperature e) repeating steps d) and e) 0 to 6 times f) if chorionic membrane is involved peeling the stromal layer from the trophoblastic layer of the chorionic membrane of step e or f) g) digesting the fragments obtained in step e), f), or g) respectively, with collagenase for 1 to 5 hours at 33 to 42.degree. C. h) collecting AMTCs and/or CMTCs from the suspension obtained in step h).
Inventor(s): Parolini; Ornella (Brescia, IT)
Assignee: Centro di Ricerca E. Menni Fondazione Poliambulanza Istituto Ospedaliero (Brescia, IT)
Application Number:12/664,713
Patent Claims:1. A method of treating lung fibrosis in a subject, which comprises: administering to the subject a medicament including amniotic mesenchymal tissue cells (AMTC) and/or chorionic mesenchymal tissue cells (CMTC), and/or the supernatant from at least 4 day AMTC and/or CMTC culture, and/or AMTC and/or CMTC that is depleted of HLA-DR-positive cells.

2. The method of claim 1, wherein the AMTC and/or CMTC are obtained with a method comprising: a) isolating amniotic membrane and/or chorionic membrane from human placenta and/or separating amniotic and chorionic membrane; b) washing the membrane of step a) to remove contaminants; c) cutting the membrane of step b) into membrane fragments; d) incubating the membrane fragments in a medium containing dispase for about 5 minutes to about 15 minutes at about 33.degree. C. to about 42.degree. C.; e) incubating the membrane fragments in a resting solution for about 5 minutes to about 15 minute at room temperature; f) repeating steps d) and e) 0 to 6 times; g) if chorionic membrane is involved, peeling the stromal layer from the trophoblastic layer of the chorionic membrane of step e) or f); h) digesting the membrane fragments with collagenase for about 1 hour to about 5 hours at about 33.degree. C. to about 42.degree. C.; and i) collecting one or more AMTC and/or CMTC from a suspension obtained in step h).

3. The method of claim 1, wherein the AMTC and/or CMTC are obtained with a method comprising separating fresh preparations of AMTC and/or CMTC, optionally by using immobilized anti-HLA-DR antibody, and eluting HLA-DR-positive cells and/or HLA-DR-negative cells.

4. The method of claim 3, wherein separating includes first incubating AMTC and/or CMTC with anti-HLA-DR antibody coated on magnetic beads and then isolating HLA-DR-positive and/or HLA-DR-negative fractions using magnetic columns.

5. The method of claim 1, wherein the medicament is a cell therapy preparation.

6. The method of claim 1, wherein the medicament includes HLA-DR-negative cells from AMTC and/or CMTC to suppress a T-cell response.

7. The method of claim 1, wherein the medicament includes HLA-DR-positive cells from AMTC and/or CMTC to stimulate lymphocyte proliferation.

8. The method of claim 1, wherein the medicament includes a supernatant obtained from at least 4 day AMTC and/or CMTC culture.

9. The method of claim 8, wherein AMTC and/or CMTC are cultured at a density of 1.times.10.sup.6 cells/well in a final volume of 1 mL of RPMI complete medium, and wherein the supernatant is collected after at least 4 days of culture, centrifuged, and filtered.

10. The method of claim 8, wherein the supernatant is obtained from at least 4 day culture of one or more fragments of amniotic membrane and/or chorionic membrane.

11. The method of claim 1, wherein the medicament is administered by an intrapulmonary route.

12. The method of claim 1, wherein the medicament is administered by an intrabronchial route.

13. The method of claim 1, wherein the medicament is administered by an intratracheal route.

14. The method of claim 1, wherein the medicament is administered by an intrajugular route.

15. The method of claim 1, wherein the medicament is administered by an intraperitoneal route.

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