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Last Updated: March 28, 2024

Claims for Patent: 8,502,026


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Summary for Patent: 8,502,026
Title:Transgenic plants with enhanced agronomic traits
Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein, enhanced seed oil and modified oil composition. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
Inventor(s): Daley; Maureen (Sacramento, CA), Fenner; Jason (Sacramento, CA), Savidge; Beth (Davis, CA), Val; Dale (Zamora, CA), Zheng; Wei (Davis, CA)
Assignee: Monsanto Technology LLC (St. Louis, MO)
Application Number:12/860,188
Patent Claims:1. A recombinant DNA molecule comprising a promoter that is functional in a plant cell and that is operably linked to a polynucleotide that, encodes a protein: i) having the ammo acid sequence of SEQ ID NO:5; or ii) having an amino acid sequence having at least 95% identity to SEQ ID NO:5 over its full length, wherein the sequence exhibits diacylglycerol acyltransferase activity.

2. The recombinant DNA molecule of claim 1 wherein said molecule further comprises at least one regulatory element selected from the group consisting of a 5' untranslated region, intron, ' untranslated region, and a transit peptide region.

3. A transgenic plant cell comprising a recombinant DNA molecule comprising a promoter that functional in a plant cell and that is operably linked to as polynucleotide that encodes a protein: i) having the amino acid sequence of SEQ ID NO:5; or ii) having an amino acid sequence having at least 95% identity to SEQ ID NO:5 over its full length, wherein the sequence exhibits diacylglycerol acyltransferase activity.

4. The transgenic plant cell of claim 3 wherein said recombinant DNA molecule is stably integrated into a chromosome in a plant cell nucleus.

5. The transgenic plant cell of claim 3 wherein said plant cell is selected by screening a population of transgenic plant cells that have been transformed with said molecule for an enhanced trait as compared to control plant cells; and wherein said enhanced trait is [enhanced water use efficiency, enhanced cold tolerance,] increased yield, [enhanced nitrogen use efficiency, enhanced seed protein,] enhanced seed oil, or modified oil composition.

6. The transgenic plant cell of claim 3 further comprising a DNA molecule expressing a protein that provides tolerance from exposure to a herbicide that is lethal to a wild type of said plant cell.

7. The transgenic plant cell of claim 6 wherein said herbicide comprises a glyphosate, dicamba, or glufosinate compound.

8. The transgenic plant cell of claim 3 wherein said plant cell is part of a transgenic plant.

9. The transgenic plant cell of claim 3 wherein said plant cell is in a plant seed.

10. The transgenic plant cell of claim 9 wherein said seed is from a corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet plant.

11. The transgenic plant cell of claim 3 further comprising at least one DNA molecule expressing a protein that provides an enhanced trait as compared to control plant cells; and wherein the enhanced trait is [enhanced water use efficiency, enhanced cold tolerance,] increased yield, [enhanced nitrogen use efficiency, enhanced seed protein,] enhanced seed oil, or modified oil composition.

12. The transgenic plant cell of claim 6 further comprising at least one DNA molecule expressing a protein that provides an enhanced trait as compared to control plant cells; and wherein the enhanced trait is [enhanced water use efficiency, enhanced cold tolerance,] increased yield, [enhanced nitrogen use efficiency, enhanced seed protein,] enhanced seed oil, or modified oil composition.

13. The transgenic plant cell of claim 7 further comprising at least one DNA molecule expressing a protein that provides an enhanced trait as compared to control plant cells; and wherein the enhanced trait is [enhanced water use efficiency, enhanced cold tolerance,] increased yield, [enhanced nitrogen use efficiency, enhanced seed protein,] enhanced seed oil, or modified oil composition.

14. A method for manufacturing non-natural, transgenic plants that can be used to produce a crop of transgenic plant with an enhanced trait resulting from expression of a stably-integrated, recombinant DNA molecule comprising a promoter that is functional in a plant and that is operably linked to a polynucleotide that[, when expressed in a plant that] encodes a protein: i) having the amino acid sequence of SEQ ID NO:5; or ii) having an amino acid sequence having at least 90% identity to SEQ ID NO:5, wherein the sequence exhibits diacylglycerol acyltransferase activity said method comprising: (a) obtaining a population of plants transformed with said recombinant DNA molecule; (b) screening the population of plants for said enhanced trait and said recombinant DNA molecule, wherein individual plants in said population exhibit said trait at a level less than, essentially the same as, or greater than the level that said trait is exhibited in control plants which do not contain said recombinant DNA molecule, wherein said enhanced trait is selected from the group of enhanced traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein, enhanced seed oil, and modified oil composition; (c) selecting from said population one or more plants that exhibit said trait at a level greater than the level that said trait is exhibited in control plants, and (d) collecting seed from the selected plant or plants from step c [b].

15. The method of claim 14 wherein said method for manufacturing said transgenic seed further comprises: (a) verifying that said recombinant DNA is stably integrated in said selected plants, and (b) analyzing tissue of said selected plant to determine the expression of a protein having the function of a protein having the amino acid sequence of SEQ ID NO:5.

16. The method of claim 15 wherein said seed is corn, soybean, cotton, canola, alfalfa, wheat, rice, sugarcane, or sugar beet seed.

17. A recombinant nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide having diacylglycerol acyltransferase activity, wherein the nucleic acid molecule is a nucleic acid sequence that encodes a polypeptide comprising a sequence that is at least 95% identical to SEQ ID NO: 5 over its full length.

18. The recombinant, nucleic acid molecule of claim 17, wherein the polypeptide comprises the sequence of SEQ ID NO: 5.

19. The recombinant nucleic acid molecule of claim 17, defined as comprising the nucleic acid sequence of SEQ ID NO: 2.

20. The transgenic plant cell of claim 3 wherein said plant cell is in an algae.

21. Oil derived from the transgenic plant cell of claim 3 that comprises said recombinant DNA molecule.

22. Oil derived from the transgenic plant cell of claim 6 that comprises said recombinant DNA molecule.

23. Oil derived from the transgenic plant cell of claim 7 that comprises said recombinant DNA molecule.

24. Oil derived from the transgenic plant cell of claim 11 that comprises said recombinant DNA molecule.

25. Oil derived from the transgenic plant cell of claim 12 that comprises said recombinant DNA molecule.

26. Oil derived from the transgenic plant cell of claim 13 that comprises said recombinant DNA molecule.

27. Oil derived from the transgenic plant cell of claim 20 that comprises said recombinant DNA molecule.

Details for Patent 8,502,026

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2029-08-24
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2029-08-24
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2029-08-24
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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