Claims for Patent: 8,323,972

Summary for Patent: 8,323,972

Title:	Mammary artery derived cells and methods of use in tissue repair and regeneration
Abstract:	An isolated mammalian internal mammary artery-derived cell is disclosed. Furthermore, methods of isolating the mammalian internal mammary artery-derived cell are disclosed. The cell is useful in tissue engineering technologies, specifically in vascular tissue engineering.
Inventor(s):	Colter; David C. (Hamilton, NJ), Buensuceso; Charito (North Brunswick, NJ), Kazanecki; Christian C. (Martins Creek, PA), Gosiewska; Anna (Skillman, NJ)
Assignee:	Advanced Technologies and Regenerative Medicine, LLC (Raynham, MA)
Application Number:	12/885,855
Patent Claims:	1. A method of isolating a mammalian internal mammary artery-derived cell comprising the steps of: a. Providing a mammalian internal mammary artery, b. providing an enzyme mixture wherein the enzyme mixture is comprised of digestion enzymes selected from the group consisting of a metalloprotease enzyme, a neutral protease enzyme, a mucolytic enzyme, and combinations thereof, c. incubating the entire mammalian internal mammary artery in the enzyme mixture for a time sufficient to remove the intimal layer and to remove external debris from the artery and provide a partially digested artery, d. transferring the partially digested artery into a fresh enzyme mixture and continue digestion for a time sufficient to provide a digested material, e. isolating the cells from the digested material, wherein the cells are positive for the expression of cell-surface marker HLA-1 and negative for expression of each one of the cell-surface markers CD 10, CD31, CD34, CD45, CD133, CD141 and KDR/Flk-1, and f. culturing the cells on collagen coated tissue culture vessels. 2. The method of claim 1 wherein the enzyme mixture is comprised of collagenase and dispase. 3. The method of claim 1 wherein the time sufficient to remove the intimal layer and to remove external debris from the artery is about 60 minutes. 4. The method of claim 1 wherein the time sufficient to provide the digested material is in the range of about 30 minutes to about 60 minutes. 5. The method of claim 4 wherein the time sufficient to provide the digested material is about 60 minutes.

Details for Patent 8,323,972

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2039-03-29