Claims for Patent: 8,283,118

Summary for Patent: 8,283,118

Title:	Isolated genomic polynucleotide fragments from chormosome 19 that encode human syntaxin binding protein 2
Abstract:	The invention is directed to isolated genomic polynucleotide fragments that encode human resistin and human syntaxin binding protein 2, vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human resistin and human syntaxin binding protein 2 and to diagnose, treat, prevent and/or ameliorate a pathological disorder.
Inventor(s):	Ryan; James (Augusta, GA)
Assignee:	Ryogen LLC (Suffern, NY)
Application Number:	13/244,497
Patent Claims:	1. An isolated nucleic acid molecule consisting of a non-coding region of a nucleic acid molecule consisting of a nucleic acid sequence which has at least 99% identity to the nucleic acid molecule of SEQ ID NO:4 and which encodes a polypeptide having the amino acid sequence of SEQ ID NO:2, wherein said polypeptide binds to syntaxins 1A, 2 and 3 but not to syntaxin 4, which non-coding region is selected from the group consisting of a 5'-noncoding region shown in sequence segment 19052-64700 of SEQ ID NO:4, a 3'-non coding region shown in sequence segment 1-8391 of SEQ ID NO:4 and an intron region shown in sequence segments 17477-19015, 17183-17425, 16472-17101, 15471-16393, 15303-15392, 14503-15203, 14167-14346, 13999-14082, 13775-13869, 13435-13666, 13222-13377, 13037-13152, 11591-12958, 11006-11449, 9953-10894, 9040-9856, 8850-8955 and 8478-8690 of SEQ ID NO:4, or a full complement of said isolated nucleic acid molecule. 2. A composition comprising the nucleic acid molecule of claim 1 and a carrier. 3. The isolated nucleic acid molecule of claim 1, wherein aid isolated nucleic acid molecule is a DNA or RNA sequence. 4. A kit comprising the nucleic acid molecule of claim 1. 5. The kit according to claim 4, in which the nucleic acid molecule is labeled with a detectable substance. 6. A microarray comprising one or more of the nucleic acid molecules of claim 1. 7. A kit comprising the microarray of claim 6. 8. A method of detecting the presence or absence of a nucleic acid sequence of SEQ ID NO:4, its complementary sequence or unique fragment thereof, said method comprising contacting the sample with the nucleic acid molecule of claim 1 and determining whether the nucleic acid molecule binds to said nucleic acid sequence in the sample. 9. A method of identifying one or more variants of SEQ ID NO:4 comprising: (a) isolating genomic DNA from a subject and (b) determining the presence or absence of said variants in said genomic DNA using the nucleic acid molecule of claim 1. 10. An isolated nucleic acid molecule consisting of a fragment of a nucleic acid molecule consisting of a nucleic acid sequence which has at least 99% identity to the nucleic acid molecule of SEQ ID NO:4 and which encodes a polypeptide having the amino acid sequence of SEQ ID NO:2, wherein said polypeptide binds to syntaxins 1A, 2 and 3 but not to syntaxin 4, wherein said fragment consists of a nucleic acid sequence selected from the group consisting of: (a) a sequence of at least 4000 contiguous nucleotides within a region consisting of the sequence of nucleotides from position 19052-64700 of SEQ ID NO:3 (b) a sequence of a least 600 contiguous nucleotides with the region consisting of the sequence of nucleotides from position 1-8390 of SEQ ID NO:3 or a full complement of said isolated nucleic acid molecule and (c) a full complement of (a) or (b). 11. A composition comprising the nucleic acid molecule of claim 10 and a carrier. 12. The isolated nucleic acid molecule of claim 10, wherein aid isolated nucleic acid molecule is a DNA or RNA sequence. 13. A kit comprising the nucleic acid molecule of claim 10. 14. The kit according to claim 13, in which the nucleic acid molecule is labeled with a detectable substance. 15. A microarray comprising one or more of the nucleic acid molecules of claim 10. 16. A kit comprising the microarray of claim 15. 17. A method of detecting the presence of a nucleic acid sequence of SEQ ID NO:4, its complementary sequence or unique fragment thereof in a sample, said method comprising contacting the sample with the nucleic acid molecule of claim 10 and determining whether the nucleic acid molecule binds to said nucleic acid sequence in the sample. 18. A method of identifying one or more variants of SEQ ID NO:4 comprising: (a) isolating genomic DNA from a subject and (b) determining the presence or absence of said variants in said genomic DNA using the nucleic acid molecule of claim 10. 19. An isolated nucleic acid molecule consisting of 20-300 contiguous nucleotides in sequence segments of a non-coding region of a nucleic acid molecule consisting of a nucleic acid sequence which has at least 99% identity to the nucleic acid molecule of SEQ ID NO:4 and which encodes a polypeptide having the amino acid sequence of SEQ ID NO:2, wherein said polypeptide binds to syntaxins 1A, 2 and 3 but not to syntaxin 4, which non-coding region is selected from the group consisting of a 5'-noncoding region shown in sequence segment 19052-64700 of SEQ ID NO:4, a 3'-non coding region shown in sequence segment 1-8391 of SEQ ID NO:4 and an intron region shown in sequence segments 17477-19015, 17183-17425, 16472-17101, 15471-16393, 15303-15392, 14503-15203, 14167-14346, 13999-14082, 13775-13869, 13435-13666, 13222-13377, 13037-13152, 11591-12958, 11006-11449, 9953-10894, 9040-9856, 8850-8955 and 8478-8690 of SEQ ID NO:4, or a full complement of said isolated nucleic acid molecule. 20. A composition comprising the nucleic acid molecule of claim 19 and a carrier. 21. The isolated nucleic acid molecule of claim 19, wherein aid isolated nucleic acid molecule is a DNA or RNA sequence. 22. A kit comprising the nucleic acid molecule of claim 19. 23. The kit according to claim 22, in which the nucleic acid molecule is labeled with a detectable substance. 24. A microarray comprising one or more of the nucleic acid molecules of claim 19. 25. A kit comprising the microarray of claim 24. 26. A method of detecting the presence of a nucleic acid sequence of SEQ ID NO:4, its complementary sequence or unique fragment thereof in a sample, said method comprising contacting the sample with the nucleic acid molecule of claim 19 and determining whether the nucleic acid molecule binds to said nucleic acid sequence in the sample. 27. A method of identifying one or more variants of SEQ ID NO:4 comprising: (a) isolating genomic DNA from a subject and (b) determining the presence or absence of said variants in said genomic DNA using the nucleic acid molecule of claim 19.

Details for Patent 8,283,118

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2025-07-09
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2025-07-09
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2025-07-09