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Last Updated: March 28, 2024

Claims for Patent: 8,185,323


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Summary for Patent: 8,185,323
Title:Method of identifying exons and introns and diagnostic uses thereof
Abstract: Novel introns and exons are detected by selecting for a specific group of known correlating splice junctions, then searching for unknown introns and exons within that group. Also, diseases are detected by selecting for introns and exons associated with diseases or mutations. Detections are achieved using comparative genomics, gel electrophoresis or protein chip technologies.
Inventor(s): Zhuo; Degen (Chapel Hill, NC)
Assignee:
Application Number:12/006,898
Patent Claims:1. A method of detecting certain exons and introns in genome data comprising the steps of: a) providing a computer for data identification and comparison purposes, the computer having access to predetermined genome data including nucleotide sequence data, predetermined 5' and 3' splicing junction data, and exon and intron data; b) obtaining genome data from biochemical analysis of a test sample or from a database; c) computer mediated identification of 5' and 3' splicing junctions in the test sample based on similarities with the predetermined 5' and 3' splicing junctions, wherein the 5' and 3' splicing junctions in the test sample have less than 100% homology with the predetermined 5' and 3' splicing junctions; and d) detecting introns and exons of the 5' and 3' splicing junctions in the test sample based on similarities with the introns and exons of the predetermined 5' and 3' splicing junctions.

2. The method of claim 1 wherein the step of identifying 5' and 3' splicing junctions in the test sample comprises the step of identifying 5' and 3' splicing junctions in DNA of the test sample by biochemical analysis.

3. The method of claim 1 wherein the step of identifying 5' and 3' splicing junctions in the test sample comprises the step of identifying 5' and 3' splicing junctions in mRNA of the test sample by biochemical analysis.

4. The method of claim 1 wherein the step of identifying 5' and 3' splicing junctions comprises the step of selecting oligomers which reflect the similarities with 6-15 base pair oligomers of predetermined 5' and 3' splicing junctions.

5. The method of claim 1 wherein the step of identifying 5' and 3' splicing junctions in the test sample comprises the step of employing comparative genomic techniques.

6. The method of claim 5 wherein the step of identifying 5' and 3' splicing junctions in the test sample employing comparative genomic techniques comprises the step of identifying 5' and 3' splicing junctions in the test sample which have at least 99% homology with predetermined 5' and 3' splicing junctions.

7. The method of claim 5 wherein the step of identifying 5' and 3' splicing junctions in the test sample employing comparative genomic techniques comprises the step of identifying 5' and 3' splicing junctions in the test sample which have at least 90% homology with predetermined 5' and 3' splicing junctions.

8. The method of claim 5 wherein the step of identifying 5' and 3' splicing junctions in the test sample employing comparative genomic techniques comprises the step of identifying 5' and 3' splicing junctions in the test sample which have at least 75% homology with predetermined 5' and 3' splicing junctions.

9. The method of claim 5 wherein the step of identifying 5' and 3' splicing junctions in the test sample employing comparative genomic techniques comprises the step of identifying 5' and 3' splicing junctions in the test sample which have at least 50% homology with predetermined 5' and 3' splicing junctions.

10. The method of claim 1 wherein said step of identifying 5' and 3' splicing junctions in the test sample comprises the step of employing electrophoretic techniques.

11. The method of claim 1 further comprising the step of correlating test sample exons and introns with exons and introns associated with diseases.

12. A method of detecting certain exons and introns in genome data comprising the steps of: a) providing a computer for data identification and comparison purposes, the computer having access to predetermined genome data including nucleotide sequence data, predetermined 5' and 3' splicing junction data, and exon and intron data; b) obtaining genome data from biochemical analysis of a test sample or from a database, selected from the group consisting of DNA and mRNA; c) computer mediated identification of 5' and 3' splicing junctions in the test sample by comparative genomic techniques based on similarities with the predetermined 5' and 3' splicing junctions, wherein the 5' and 3' splicing junctions in the test sample have less than 100% homology with the predetermined 5' and 3' splicing junctions; and d) detecting introns and exons of the 5' and 3' splicing junctions in the test sample based on similarities with the introns and exons of the predetermined 5' and 3' splicing junctions.

13. The method of claim 12 further comprising the step of correlating test sample exons and introns with exons and introns associated with diseases.

14. The method of claim 13 further comprising the step of predicting increased susceptibility to the diseases based on the correlation.

15. The method of claim 13 further comprising the step of diagnosing the diseases based on the correlation.

Details for Patent 8,185,323

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2039-02-26
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2039-02-26
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2039-02-26
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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