Claims for Patent: 8,105,380

Summary for Patent: 8,105,380

Title:	Cellular scaffold
Abstract:	A cellular scaffold that is suitable for tissue regeneration, cell culture and in vitro assays. The invention relates to a layered cell scaffold that is seeded with mesenchymal and ectodermal cells. The layered cellular scaffold comprises an inoculum of mesenchymal cells and ectodermal cells positioned between two opposing scaffolds in a sandwich configuration. The layered cell scaffold provides a functional skin equivalent that is suitable for transplantation and in vitro cell-based assays.
Inventor(s):	Kharazi; Alexander (San Diego, CA), Kharazi; Ludmila (San Diego, CA)
Assignee:	Stemedica Cell Technologies, Inc. (San Diego, CA)
Application Number:	11/616,787
Patent Claims:	1. A cellular scaffold arrangement comprising: a first collagen cell scaffold having a first surface and a second surface wherein said second surface of said first cell scaffold is seeded with mesenchymal cells; and a second collagen cell scaffold having a first surface and a second surface wherein said first surface of said second cell scaffold is seeded with ectodermal cells; wherein said second surface of said first scaffold is in contact with said first surface of said second scaffold; wherein said second cell scaffold has the consistency of a sponge. 2. The cellular scaffold-of claim 1, wherein said first cell scaffold has a consistency selected from a sponge, a membrane, a gel, and combinations thereof. 3. The cellular scaffold of claim 1, wherein said mesenchymal cells are derived from at least one of umbilical cord blood, placenta, bone marrow and peripheral blood. 4. The cellular scaffold of claim 1, wherein said mesenchymal cells comprise fibroblasts. 5. The cellular scaffold of claim 1, wherein said ectodermal cells comprise precursor keratinocytes. 6. The cellular scaffold of claim 1, wherein said ectodermal cells are derived from basal keratinocytes. 7. The cellular scaffold of claim 5, wherein said precursor keratinocytes are inhibited in collagenase production. 8. A layered cellular scaffold comprising: a first collagen cellular scaffold having a contacting surface that is seeded with a plurality of cells comprising ectodermal cells; a second collagen cellular scaffold having a contacting surface that is seeded with mesenchymal cells; and a scaffold interface wherein said first scaffold contacting surface substantially contacts said second scaffold contacting surface; wherein said first cellular scaffold has the consistency of a sponge. 9. The layered cellular scaffold of claim 8, wherein said second cellular scaffold has a consistency selected from a sponge, a membrane, a gel, and combinations thereof. 10. The layered cellular scaffold of claim 8, wherein said mesenchymal cells are derived from at least one of umbilical cord blood, placenta, bone marrow and peripheral blood. 11. The layered cellular scaffold of claim 8, wherein said mesenchymal cells comprise fibroblasts. 12. The layered cellular scaffold of claim 8, wherein said ectodermal cells comprise precursor keratinocytes. 13. The layered cellular scaffold of claim 8, wherein said ectodermal cells are derived from basal keratinocytes. 14. The layered cellular scaffold of claim 12, wherein said precursor keratinocytes are inhibited in collagenase production. 15. A method of making a layered cellular scaffold comprising: providing a first cell substrate having a first surface and a second surface; seeding said second surface of said first cell substrate with ectodermal cells; providing a second cell substrate having a first surface and a second surface; seeding said first surface of said second cell substrate with mesenchymal cells; and contacting said second surface of said first cell substrate with said first surface of said second cell substrate thereby producing said layered cellular scaffold; wherein said first and second cell substrates comprise collagen; and wherein said first cell substrate has the consistency of a sponge. 16. The method of claim 15, wherein said second cell substrate has a consistency selected from a sponge, a membrane, a gel, and combinations thereof. 17. The method of claim 15, wherein said mesenchymal cells are derived from at least one of umbilical cord blood, placenta, bone marrow and peripheral blood. 18. The method of claim 15, wherein said mesenchymal cells are fibroblasts. 19. The method of claim 15, wherein said ectodermal cells are precursor keratinocytes. 20. The method of claim 15, wherein said ectodermal cells are derived from basal keratinocytes. 21. The method of claim 19, wherein said precursor keratinocytes are inhibited in collagenase production. 22. A method for making a layered cellular scaffold comprising: providing a first cell scaffold and a second cell scaffold; seeding a surface of said first cell scaffold with mesenchymal cells; seeding a surface of said second cell scaffold with ectodermal cells; and substantially joining said seeded surfaces to create said layered cellular scaffold; wherein said first and said second cell scaffolds comprise collagen; and wherein said second cell scaffold has the consistency of a sponge. 23. The method of claim 22, wherein said first cell scaffold has a consistency selected from a sponge, a membrane, a gel, and combinations thereof. 24. The method of claim 22, wherein said mesenchymal cells are derived from at least one of umbilical cord blood, placenta, bone marrow and peripheral blood. 25. The method of claim 22, wherein said mesenchymal cells are fibroblasts. 26. The method of claim 22, wherein said ectodermal cells are precursor keratinocytes. 27. The method of claim 22, wherein said ectodermal cells are derived from basal keratinocytes. 28. The method of claim 26, wherein said precursor keratinocytes are inhibited in collagenase production.

Details for Patent 8,105,380

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2026-10-23