Claims for Patent: 7,985,408

Summary for Patent: 7,985,408

Title:	Tenocyte cell culturing method
Abstract:	The present invention relates to a method for culturing tenocytes. In particular the present invention relates to a method for culturing tenocytes comprising the step of incubating tenocytes in a culture medium comprising insulin or functional derivative.
Inventor(s):	Zheng; Ming Hao (City Beach, AU)
Assignee:	The University of Western Australia (Nedlands, AU)
Application Number:	12/294,169
Patent Claims:	1. A tenocyte culture medium comprising insulin and betamethasone. 2. The tenocyte culture medium of claim 1, which comprises about 0.00005% to about 0.1% w/v insulin. 3. The tenocyte culture medium of claim 1, which comprises about 0.0001% to about 0.001% w/v insulin. 4. The tenocyte culture medium of claim 1, which comprises about 0.0006% w/v insulin. 5. The tenocyte culture medium of claim 1, which comprises about 0.00001% to about 0.1% w/v betamethasone. 6. A method of culturing a tenocyte, comprising the step of incubating a tenocyte or tissue containing a tenocyte in the culture medium of claim 1. 7. A method for producing a culture of tenocytes comprising the steps of: (i) isolating a tenocyte-containing tissue, washing and mincing same to form explants; and (ii) culturing said explants in the presence of a tenocyte culture medium comprising insulin and betamethasone to yield free tenocytes; wherein at least 90% of cells present in the culture are tenocytes. 8. The method of claim 7, wherein at least 95% of total cells present in the culture are tenocytes. 9. The tenocyte culture medium of claim 1, which further comprises serum, one or more antibiotics, L-proline or combinations thereof. 10. The tenocyte culture medium of claim 9, wherein the serum is fetal bovine serum. 11. The tenocyte culture medium of claim 9, wherein the antibiotic is selected from one or more of the group consisting of ampicillin, carbenicillin, penicillin, amphotericin, nystatin, polymixin-B, gentamycin, kanarnycin, neomycin, streptomycin, a tetracycline, a macrolide, linomycin, tiamutin, and chloramphenicol. 12. The method of claim 7, wherein the tenocyte-containing tissue is tendon tissue. 13. The method of claim 7, wherein the tenocyte-containing tissue is minced to no larger than 1 mm and is incubated in the presence of 2.5% w/v trypsin and 5.5% w/v collagenase. 14. A tenocyte seeded support matrix comprising a support matrix and a substantially pure culture of tenocytes; where said tenocytes are produced by the method of claim 7 and wherein said tenocytes are seeded onto said support matrix until said tenocytes adhere to the support matrix.

Details for Patent 7,985,408

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2026-03-23