Claims for Patent: 7,982,092

Summary for Patent: 7,982,092

Title:	Chimeric gene comprising intron from histone H3.3 gene
Abstract:	An isolated DNA sequence capable of serving as regulatory element in a chimeric gene which can be used for the transformation of plants is disclosed. A chimeric gene containing the DNA sequence as a regulatory element for the transformation of plants is also disclosed. The gene comprises at least, in the direction of transcription, a promoter sequence, a transgene and a regulatory element, characterized in that the regulatory element comprises an intron from the noncoding 5\' region of a plant histone gene, allowing the expression of the protein in the zones undergoing rapid growth. The production of transgenic plants is also disclosed.
Inventor(s):	Derose; Richard (Lyons, FR), Chaubet, legal reprsentative; Nicole (Strasbourg, FR), Gigot; Claude (Strasbourg, FR)
Assignee:	Bayer Cropscience SA (Lyons, FR)
Application Number:	11/797,078
Patent Claims:	1. A method for preferentially expressing a chimeric gene in rapidly growing tissues of a transgenic plant, said method comprising transfecting plant cells with a chimeric gene comprising the first intron of the 5' non-translated region of a plant H3.3 histone gene as a regulatory element, and thereby causing the expression of said chimeric gene in rapidly growing tissues of said transgenic plant. 2. A method of making a chimeric gene comprising: obtaining a DNA molecule comprising a promoter sequence; obtaining a DNA molecule comprising an intron from the 5' non-coding region of a plant histone H3.3-like gene; obtaining a DNA molecule encoding a signal peptide sequence; obtaining a DNA molecule comprising a sequence encoding an enzyme; constructing the chimeric gene by linking the promoter sequence, the intron derived from the 5' non-coding region of a plant histone H3.3-like gene, the DNA encoding the signal peptide sequence and the sequence encoding an enzyme, optionally including one or more linker sequences. 3. The method of claim 2, wherein the sequence encoding an enzyme encodes a herbicide tolerance enzyme. 4. The method of claim 2, wherein the sequence encoding an enzyme encodes an EPSPS enzyme. 5. The method of claim 2, wherein obtaining a DNA molecule comprising an intron from the 5' non-coding region of a plant histone H3.3-like gene comprises isolating an intron from the 5' non-coding region of a gene in a plant genomic library that hybridizes under stringent conditions with a labeled histone H3.3 coding region probe. 6. The method of claim 5, wherein the plant is selected from among wheat maize or rice. 7. The method of claim 5, wherein the plant is selected from among lucerne, sunflower, soya bean, rapeseed, or Arabidopsis thaliana. 8. The method of claim 2, wherein the DNA molecule comprising an intron from the 5' non-coding region of a plant histone H3.3-like gene comprises a DNA sequence as described in SEQ ID NO: 6 or SEQ ID NO: 7. 9. A method of making a transgenic plant comprising obtaining a chimeric gene according to the method of claim 2, stably transforming plant cells with a DNA molecule comprising the chimeric gene, and growing a transgenic plant from said transformed plant cells. 10. A chimeric gene made by the method of claim 2. 11. A plant comprising as a transgene a chimeric gene made by the method of claim 2. 12. A chimeric gene comprising a promoter, an intron derived from the 5' non-translated region of a plant H3.3 histone gene, and a coding sequence.

Details for Patent 7,982,092

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2015-07-19
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2015-07-19
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2015-07-19