Claims for Patent: 7,897,850

Summary for Patent: 7,897,850

Title:	Barley Lipoxygenase 1 Gene, method of selecting barley variety, material of malt alcoholic drinks and process for producing malt alcoholic drink
Abstract:	A selection method for barley lipoxygenase-1 deficient barley, comprising a step of distinguishing the barley lipoxygenase-1 deficient barley by whether or not the guanine at the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene is mutated to a different base; and a method for production of malt alcoholic beverages using a material for malt alcoholic beverages derived from barley obtained by the selection method.
Inventor(s):	Hirota; Naohiko (Gunma, JP), Kaneko; Takafumi (Gunma, JP), Kuroda; Hisao (Yaizu, JP), Kaneda; Hirotaka (Yaizu, JP), Takoi; Kiyoshi (Yaizu, JP), Takeda; Kazuyoshi (Kurashiki, JP)
Assignee:	Sapporo Breweries Limited (Tokyo, JP)
Application Number:	10/550,528
Patent Claims:	1. An isolated barley lipoxygenase-1 mutant gene, wherein the guanine at the splicing donor site (5'-GT-3') of the 5th intron of the barley lipoxygenase-1 gene is not guanine. 2. The isolated barley lipoxygenase-1 mutant gene according to claim 1, wherein the nucleotide guanine at the splicing donor site is replaced with adenine. 3. A selection method for barley lipoxygenase-1 deficient barley, the method comprising determining the presence or absence of a guanine at the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene, and selecting the barley having an adenine, thymine or cytosine at the splicing donor site. 4. The selection method for barley lipoxygenase-1 deficient barley according to claim 3, comprising selecting the barley having an adenine at the splicing donor site. 5. The selection method for barley lipoxygenase-1 deficient barley according to claim 3 or 4, wherein the determining comprises extracting genomic DNA from a barley sample, amplifying a DNA fragment containing at least the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene from the extracted genomic DNA, and detecting the amplified DNA fragment by cleaving with a restriction enzyme to determine the presence or absence of guanine at the splicing donor of the 5.sup.th intron of the barley lipoxygenase-1 gene. 6. The selection method for barley lipoxygenase-1 deficient barley according to claim 5, wherein the restriction enzyme. 7. A material for malt alcoholic beverages, wherein the material is selected from a group consisting of a seed, a malt, malt extract, barley decomposition product or processed barley derived from barley, comprising the barley lipoxygenase-1 mutant gene-according to claim 1 or 2. 8. A material for malt alcoholic beverages, wherein the material is selected from a group consisting of a seed, a malt, malt extract, barley decomposition product or processed barley derived from barley selected by the selection method according to claim 3. 9. A method for producing malt alcoholic beverages, the method comprising fermenting wort obtained from a seed, a malt, malt extract, barley decomposition product or processed barley derived from barley according to claim 7 or 8. 10. An isolated nucleic acid comprising the nucleotide sequence from position 1 to 1554 as set forth in SEQ ID NO: 10. 11. An isolated nucleic acid comprising the nucleotide sequence as set forth in SEQ ID NO: 11. 12. An isolated nucleic acid comprising the nucleotide sequence of 10 to 60 continuous bases including the 3178th base in the nucleotide sequence as set forth in SEQ ID NO: 11. 13. A method for detecting the presence of lipoxygenase-1 (LOX-1) activity in barley, the method comprising isolating genomic DNA from a barley sample, and detecting the presence or absence of the 3178th base of SEQ ID NO: 11, wherein the presence of the base is an indicator of the presence of LOX-1 activity in the barley. 14. A seed, a malt, malt extract, barley decomposition product or processed barley derived from barley selected by the selection method according to claim 4. 15. A seed, a malt, malt extract, barley decomposition product or processed barley derived from barley selected by the selection method according to claim 5. 16. A seed, a malt, malt extract, barley decomposition product or processed barley derived from barley selected by the selection method according to claim 6. 17. A method for producing malt alcoholic beverages, the method comprising fermenting a seed, a malt, malt extract, barley decomposition product or processed barley derived from barley according to claim 14, 15, or 16.

Details for Patent 7,897,850

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2023-03-25
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2023-03-25
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2023-03-25