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Last Updated: April 23, 2024

Claims for Patent: 7,863,042

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Summary for Patent: 7,863,042

Title:	Fucose transporter
Abstract:	The present invention provides a gene encoding a fucose transporter, a fucose transporter polypeptide, a method for screening for a compound that binds to a fucose transporter or a compound that inhibits fucose transport activity, a cell having inhibited fucose transporter functions, and a cell wherein the expression of the fucose transporter is inhibited. The present invention further relates to a method for producing recombinant protein, and specifically, to a method for producing protein by which fucose existing within the Golgi apparatus of a host cell is decreased, a method for inhibiting the addition of fucose to protein by which fucose existing within the Golgi apparatus of a host cell is decreased upon production of recombinant protein using the host cell, a method for increasing the cytotoxic activity of an antibody by which an antibody is produced using a cell wherein fucose existing within the Golgi apparatus is decreased, and a cell having a Golgi apparatus wherein fucose existing within the Golgi apparatus is decreased.
Inventor(s):	Tsuchiya; Masayuki (Shizuoka, JP), Iijima; Shigeyuki (Shizuoka, JP), Sugo; Izumi (Shizuoka, JP), Sekimori; Yasuo (Shizuoka, JP), Ueno; Kenju (Shizuoka, JP), Habu; Kiyoshi (Ibaraki, JP)
Assignee:	Chugai Seiyaku Kabushiki Kaisha (Tokyo, JP)
Application Number:	10/561,191
Patent Claims:	1. An isolated Chinese hamster cell, wherein one or more exons of the genomic Chinese hamster fucose transporter gene having the sequence of SEQ ID NO: 1 are disrupted. 2. The isolated Chinese hamster cell according to claim 1, wherein the Chinese hamster cell is a Chinese hamster ovary (CHO) cell. 3. The isolated CHO cell according to claim 2, wherein one or more of the genomic Chinese hamster fucose transporter gene are disrupted by homologous recombination using a gene targeting vector against the sequence in the genomic Chinese hamster fucose transporter gene having the sequence of SEQ ID NO: 1. 4. The isolated Chinese hamster cell according to claim 3, wherein exon 1 is disrupted. 5. The isolated Chinese hamster cell according to claim 3, wherein the Chinese hamster fucose transporter gene is disrupted by a positive selection marker. 6. The isolated Chinese hamster cell according to claim 5, wherein the positive selection marker is a drug resistance gene. 7. The isolated Chinese hamster cell according to claim 5, wherein the positive selection marker is a hygromycin resistance gene or a neomycin resistance gene. 8. The isolated Chinese hamster cell according to claim 5, wherein the positive selection marker disrupts an exon. 9. The isolated Chinese hamster cell according to claim 3, wherein the gene targeting vector comprises, a positive selection marker, a first nucleotide sequence homologous to at least part of SEQ ID NO:1 that is located 5' of the positive selection marker, a second nucleotide sequence homologous to at least part of SEQ ID NO:1 that is located 3' of the positive selection marker, a first restriction enzyme site for linearizing the vector located outside the positive selection marker, the first nucleotide sequence and the second nucleotide sequence, and a second restriction enzyme site for detecting homologous recombination. 10. The isolated Chinese hamster cell according to claim 9, wherein at least one of the first nucleotide sequence and the second nucleotide sequence is homologous to at least a part of an intron. 11. The isolated Chinese hamster cell according to claim 1 or 2, wherein exon 1 is disrupted.

Details for Patent 7,863,042

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986	⤷ Try a Trial	2023-06-18
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132		⤷ Try a Trial	2023-06-18
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132		⤷ Try a Trial	2023-06-18
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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