Claims for Patent: 7,838,227

Summary for Patent: 7,838,227

Title:	Simultaneous detection of biological agents by solid-state hybridization and naked eye visualization
Abstract:	The invention relates to methods and products simultaneously enabling detection of several biological threat agents, including viruses and bacteria, during a combat situation or in any suspected contamination situation.
Inventor(s):	Sagripanti; Jose-Luis (BelAir, MD)
Assignee:	The United States of America as represented by the Secretary of the Army (Washington, DC)
Application Number:	11/546,741
Patent Claims:	1. A method of detecting a biological threat agent, comprising the steps of: (a) extracting target nucleic acid from a suspect sample; (b) labeling said target nucleic acid to produce labeled target nucleic acid; (c) exposing said labeled target nucleic acid to capture nucleic acid, wherein said capture nucleic acid comprises a plurality of individual nucleic acid sequences bound to a solid substrate, and wherein each of said individual nucleic acid sequences is positioned at a specific location on said solid substrate, wherein said capture nucleic acid comprising a plurality of individual nucleic acid sequences comprises the nucleotide sequences consisting of SEQ ID NOS: 1-48; and (d) determining the presence of a biological threat agent by reading a signal emitted by said labeled target nucleic acid after it attaches to one or more of said capture nucleic acids at specific locations on said substrate; wherein said reading a signal consists of visual inspection by the naked eye. 2. The method of claim 1, wherein the biological threat agent is selected from the group consisting of an alphavirus, orthopoxvirus, filovirus, bunyavirus, flavivirus, paramyxovirus, Bacillus anthracis, Yersinia, Francicella tularensis, Brucellas, E. coli 0157:117, Burkholderias, Coxiella burnetti, Rickettsias or a combination thereof. 3. The method of claim 1, wherein said labeling comprises attaching a label selected from the group consisting of visible, UV, IR fluorescent, radioactive, mass, and electrochemical labels. 4. The method of claim 3, wherein the label comprises digoxigenin. 5. The method of claim 1, wherein before labeling the target nucleic acid is amplified by polymerase chain reaction using primer sets comprising SEQ ID NOS: 1-48. 6. A method of detecting a biological threat agent, comprising the steps of: (a) extracting target nucleic acid from a suspect sample; (b) exposing said target nucleic acid to capture nucleic acid, wherein said capture nucleic acid comprises a plurality of individual nucleic acid sequences bound to a solid substrate, and wherein each of said individual nucleic acid sequences is positioned at a specific location on said solid substrate, wherein said capture nucleic acid comprising a plurality of individual nucleic acid sequences comprises the nucleotide sequences consisting of SEQ ID NOS: 1-48; (c) labeling said target nucleic acid to produce a labeled target nucleic acid; and (d) determining the presence of a biological threat agent by reading a signal emitted by said labeled target nucleic acid after it attaches to one or more of said capture nucleic acids at specific locations on said substrate; wherein said reading a signal consists of visual inspection by the naked eye. 7. The method of claim 6, wherein the biological threat agent is selected from the group comprising an alphavirus, orthopoxvirus, filovirus, bunyavirus, flavivirus, paramyxovirus, Bacillus anthracis, Yersinia, Francicella tularensis, Brucellas, E. coli 0157:H7, Burkholderias, Coxiella burnetti, Rickettsias or a combination thereof. 8. The method of claim 6, wherein said labeling comprises attaching a label selected from the group consisting of visible, UV, IR fluorescent, radioactive, mass, and electrochemical labels. 9. The method of claim 8, wherein the label comprises digoxigenin. 10. The method of claim 6, wherein before exposing said target nucleic acid to capture nucleic acid, the target nucleic acid is amplified by polymerase chain reaction using primers sets comprising SEQ ID NOS: 1-48. 11. A method of detecting a biological threat agent, comprising the steps of: (a) extracting target nucleic acid from a suspect sample; (b) labeling said target nucleic acid with a chromatic substance to produce a labeled target nucleic acid; (c) exposing said labeled target nucleic acid to capture nucleic acid, wherein said capture nucleic acid comprises a plurality of individual nucleic acid sequences bound to a solid substrate, and wherein each of said individual nucleic acid sequences is positioned at a specific location on said solid substrate, wherein said capture nucleic acid comprising a plurality of individual nucleic acid sequences comprises the nucleotide sequences consisting of SEQ ID NOS: 1-48; and (d) determining the presence of a biological threat agent by reading a signal emitted by said labeled target nucleic acid after it attaches to one or more of said capture nucleic acids at specific locations on said substrate; wherein said reading a signal consists of visual inspection by the naked eye. 12. The method of claim 11, wherein the biological threat agent is selected from the group consisting of an alphavirus, orthopoxvirus, filovirus, bunyavirus, flavivirus, paramyxovirus, Bacillus anthracis, Yersinia, Francicella tularensis, Brucellas, E. coli 0157:H7, Burkholderias, Coxiella burnetti, Rickettsias or a combination thereof. 13. The method of claim 11, wherein the visual inspection by the naked eye comprises identification of colors on the solid substrate. 14. The method of claim 11, wherein before labeling the target nucleic acid is amplified by polymerase chain reaction using primer sets comprising SEQ ID NOS: 1-48.

Details for Patent 7,838,227

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Emergent Biodefense Operations Lansing Llc	BIOTHRAX	anthrax vaccine adsorbed	Injection	103821	11/12/1998		2025-10-24