Claims for Patent: 7,838,053

Summary for Patent: 7,838,053

Title:	Barley for production of flavor-stable beverage
Abstract:	According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.
Inventor(s):	Breddam; Klaus (Osted, DK), Olsen; Ole (Copenhagen S., DK), Skadhauge; Birgitte (Birkerod, DK), Lok; Finn (Valby, DK), Knudsen; Soren (Malov, DK), Bech; Lene Molskov (Smorum, DK)
Assignee:	Carlsberg A/S (Valby, DK)
Application Number:	10/598,779
Patent Claims:	1. A plant product produced from a barley plant, or a part thereof, wherein the barley plant has a mutation in the LOX-1 gene so that it encodes a mutated LOX-1 protein lacking all or at least a portion of amino acids 520 to 862 of wild type barley LOX-1 (SEQ ID NO: 3 or 7) with a total loss of LOX-1 activity. 2. The plant product of claim 1, wherein said plant product is a wort composition prepared from: a) the barley plant or part thereof; or b) a malt composition prepared from said barley plant or part thereof; or c) a mixture of a) and b). 3. The plant product according to claim 2, wherein the plant product is a wort composition, and wherein said part of said plant is kernel(s). 4. The plant product according to claim 2, wherein the plant product is a wort composition and wherein said malt composition is a malt composition comprising a processed barley plant or part thereof. 5. The plant product according to claim 2, wherein the plant product is a wort composition, and wherein said composition is prepared further using an enzyme composition or an enzyme mixture composition. 6. The plant product of claim 1, wherein the plant product is a wort composition or a beverage prepared from a composition comprising said barley plant, or a part thereof, and a malt composition prepared from said barley plant. 7. The plant product of claim 1, wherein the plant product is a beverage having stable organoleptic qualities, wherein said beverage is obtained by manufacturing a barley plant or part thereof. 8. The plant product of claim 7, wherein said beverage is beer. 9. The plant product of claim 7, wherein said beverage is prepared using malt prepared from kernels of said barley plant. 10. The plant product of claim 7 wherein said beverage is prepared from a wort composition prepared from a barley plant or part thereof, or from a malt composition prepared from said barley plant or part thereof. 11. The plant product of claim 7, wherein said beverage is prepared from unmalted barley plants or parts thereof. 12. The plant product of claim 7, wherein said beverage is a non-fermented beverage. 13. The plant product of claim 7, wherein said barley plant, or parts thereof, comprise a LOX-1 gene, said gene comprising: (i) a nonsense codon; or (ii) a splice site mutation. 14. The plant product of claim 13, wherein the gene encoding LOX-1 comprises: (i) a nonsense codon, said codon corresponding to base nos. 3572-3574 of SEQ ID NO: 2; or (ii) a splice site mutation, said mutation corresponding to base no. 2311 of SEQ ID NO: 6. 15. A beverage having stable organoleptic qualities, wherein said beverage is manufactured by using a barley plant, wherein: the barley plant has a mutation in the LOX-1 gene so that it encodes a mutated LOX-1 protein lacking all or at least a portion of amino acids 520 to 862 of wild type barley LOX-1 (SEQ ID NO: 3 or 7) with a total loss of LOX-1 activity; the ratio of 9,12,13-trihydroxyoctadecenoic acid to 9,10,13-trihydroxyoctadecenoic acid within said beverage is at the most 1.8. 16. The beverage according to claim 15, wherein said beverage is beer. 17. The beverage of claim 15, wherein said beverage comprises at the most 0.05 ppb free trans-2-nonenal (T2N) after incubation at 37.degree. C. for 4 weeks, in the presence of in the range of 4 to 6 ppm sulfite. 18. The plant product according to claim 1, wherein said plant product is a beverage. 19. A method of producing: (i) a food composition; or (ii) a feed composition; or (iii) a fragrance raw material composition; or (iv) a malt composition; or (v) a wort composition; or (vi) a beverage; or (vii) any combination of (i) to (vi); using a barley plant or part thereof, wherein the barley plant has a mutation in the LOX-1 gene so that it encodes a mutated LOX-1 protein lacking all or at least a portion of amino acids 520 to 862 of wild type barley LOX-1 (SEQ ID NO: 3 or 7) with a total loss of LOX-1 activity. 20. The plant product of claim 1, wherein said plant product is a food composition, a feed composition, or a fragrance raw material composition comprising the barley plant or part thereof. 21. The method of claim 19 wherein said method is a method for producing a beverage having stable organoleptic qualities, said method comprising the steps of: (i) preparing a composition comprising a barley plant or parts thereof; (ii) processing the composition of (i) into a beverage; thereby obtaining a beverage with stable organoleptic qualities. 22. The method according to claim 21, wherein step (i) comprises preparing a malt composition from kernels of said barley plant or part thereof. 23. The method according to claim 21, wherein the method further comprises incubation with a LOX inhibitor. 24. The method according to claim 21, wherein processing the composition into a beverages comprises a mashing step. 25. The method according to claim 21, wherein a LOX inhibitor is added during said mashing step. 26. The plant product of claim 1, wherein the barley plant does not carry a mutation of the guanosine residue in the splice donor site of intron 5. 27. The method of claim 19, wherein the barley plant does not carry a mutation of the guanosine residue in the splice donor site of intron 5.

Details for Patent 7,838,053

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2039-03-29
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2039-03-29
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2039-03-29