Claims for Patent: 7,816,506
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Summary for Patent: 7,816,506
Title: | Methods and constructs for producing transgenic plants and methods of recovering expressed proteins |
Abstract: | This disclosure relates to a regulatory element useful for genetically engineering sugarcane or other monocots, to the transformation of the monocots with the regulatory element so that they produce a desired product, and to the regeneration of the monocots transformed with the regulatory element. In particular the present disclosure provides a nucleic acid encoding a promoter of a Sugarcane Elongation Factor (SEF), for example, as shown in SEQ ID NO: 5. |
Inventor(s): | Mirkov; T. Erik (Harlingen, TX), Paterson; Andrew (Arnoldsville, GA), Yang; Meizhu (Johnston, IA) |
Assignee: | The Texas A & M University System (College Station, TX) |
Application Number: | 12/188,069 |
Patent Claims: | 1. An isolated nucleic acid comprising a nucleic acid sequence, wherein the nucleic acid sequence comprises a promoter, wherein the promoter
comprises the sequence of nucleotides 1 to 1967 of SEQ ID NO: 5.
2. The isolated nucleic acid of claim 1 further comprises a nucleic sequence having the sequence of nucleotides 2430 to 3207 of SEQ ID NO:5. 3. The isolated nucleic acid of claim 2 wherein the nucleic sequence of nucleotides 2430 to 3207 of SEQ ID NO:5 is an intron. 4. The isolated nucleic acid of claim 1 wherein the nucleic acid is operable to express an exogenous nucleic acid in a monocot. 5. The isolated nucleic acid of claim 1 further comprising an exogenous nucleic acid under control of the promoter, wherein the isolated nucleic acid is operable to express the exogenous nucleic acid in a monocot. 6. The isolated nucleic acid of claim 5, wherein the exogenous nucleic acid comprises a transgene. 7. The isolated nucleic acid of claim 6, wherein the transgene encodes a gene product selected from the group consisting of bactericidal, insecticidal, and antiviral proteins. 8. The isolated nucleic acid of claim 7, wherein the bactericidal protein is bovine lysozyme. 9. The isolated nucleic acid of claim 7, wherein the insecticidal and antiviral protein is snow drop lectin. 10. An expression vector comprising, in a 5' to 3' direction: a promoter having the sequence of nucleotides 1 to 1967 of SEQ ID NO: 5; an intron nucleic sequence having the sequence of nucleotides 2430 to 3207 of SEQ ID NO:5; an exogenous nucleic acid; and a 3' termination sequence. 11. The expression vector of claim 10, wherein the exogenous nucleic acid comprises a transgene. 12. The expression vector of claim 11, wherein the transgene encodes a gene product selected from the group consisting of bactericidal, insecticidal, and antiviral proteins. 13. The expression vector of claim 12, wherein the bactericidal protein is bovine lysozyme. 14. The expression vector of claim 12, wherein the insecticidal and antiviral protein is snow drop lectin. 15. A method expressing of a nucleic acid in a monocot plant comprising: providing an expression nucleic acid having a promoter having the sequence of nucleotides 1 to 1967 of SEQ ID NO: 5, an exogenous nucleic acid and a 3' termination sequence; and transforming the plant with the expression nucleic acid. 16. The method of claim 15, wherein the expression nucleic acid further comprises an intron nucleic sequence having the sequence of nucleotides 2430 to 3207 of SEQ ID NO:5. 17. The method of claim 15, wherein transforming further comprises biolistically transforming the plant with the expression nucleic acid. 18. The method of claim 15, wherein transforming further comprises Agrobacterium-mediated transformation. 19. The method of claim 15, wherein the plant comprises an embryonic callus. 20. The method of claim 19 further comprising regenerating a plant from the embryonic callus. 21. An isolated nucleic acid comprising a nucleic acid sequence, wherein the nucleic acid sequence comprises the sequence of nucleotides 1 to 1967 of SEQ ID NO: 5. |
Details for Patent 7,816,506
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | 06/04/1986 | ⤷ Try a Trial | 2020-04-11 |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | ⤷ Try a Trial | 2020-04-11 | |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | Injection | 103132 | ⤷ Try a Trial | 2020-04-11 | |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
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