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Last Updated: March 28, 2024

Claims for Patent: 7,754,861


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Summary for Patent: 7,754,861
Title:Method for purifying proteins
Abstract: The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moieties having different binding moieties, binding the contaminating proteins and a minority of the target protein group to the library of binding moieties, separating the unbound target protein group from the proteins bound to the library of binding moieties and collecting the unbound target protein. The collected target protein is more pure than the target protein group in the sample.
Inventor(s): Boschetti; Egisto (Croissy sur Seine, FR), Lomas; Lee O. (Pleasanton, CA)
Assignee: Bio-Rad Laboratories, Inc. (Hercules, CA)
Application Number:11/388,181
Patent Claims:1. A method for purifying a target protein group comprising the steps of: (a) contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library comprising at least 100 different binding moieties in an amount sufficient to bind a majority of the contaminating proteins and a minority of the target protein group, but not a majority of the target protein group; (b) binding the majority of the contaminating proteins and the minority of the target protein group, but not the majority of the target protein group, to the library of binding moieties; (c) separating the unbound majority of the target protein group from the majority of the contaminating proteins and the minority of the target protein group bound to the library of binding moieties; and (d) collecting the unbound majority of the target protein group which is more pure than the target protein group in the sample.

2. The method according to claim 1, wherein the sample comprises at least 98% of the target protein group and at most 2% of contaminating proteins.

3. The method according to claim 1, wherein the sample comprises a fermentation broth.

4. The method according to claim 1, wherein the target protein group consists of a single protein species.

5. The method according to claim 1, wherein the target protein group comprises a recombinant protein.

6. The method according to claim 5, wherein the recombinant protein is selected from the group consisting of an enzyme, a hormone, a growth factor, a receptor, a vaccine, an immunoglobulin and fragments of any of the foregoing.

7. The method according to claim 5, wherein the recombinant protein is selected from the group consisting of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), epidermal growth factor (EGF), transforming growth factor (TGF), insulin-like growth factor (IGF), erythropoietin (EPO), bone morphogenetic protein (BMP), bone-derived neurotrophic factor (BDNF), colony stimulating factor (CSF), nerve growth factor (NGF), human growth hormone (hGH), tumor necrosis factor (TNF), insulin, tissue-type plasminogen activator (t-PA), interferon, interleukin (IL) and herceptin.

8. The method according to claim 1, wherein the target protein group comprises a naturally occurring protein.

9. The method according to claim 1, wherein the library comprises at least 1,000, at least 10,000, at least 100,000, at least 1,000,000, at least 10,000,000 or at least 100,000,000 different binding moieties.

10. The method according to claim 1, wherein the library is a non-selective library selected from the group consisting of a germ line antibody library, a phage display library of recombinant polypeptides, a dye library, a non-combinatorial library, a combinatorial library and portions of any of the foregoing.

11. The method according to claim 10, wherein the library comprises at least a portion of a combinatorial library.

12. The method according to claim 11, wherein the combinatorial library comprises binding moieties selected from polypeptides, peptides, hexapeptides, polynucleotides, lipids, oligosaccharides and small organic molecules.

13. The method according to claim 11, wherein the combinatorial library is a combinatorial library of hexapeptides.

14. The method according to claim 1, wherein the binding moieties comprise bio-organic polymers.

15. The method according to claim 1, wherein the binding moieties are selected from the group consisting of dyes, polypeptides, peptides, hexapeptides, antibodies, nucleic acids, aptamers and small organic molecules.

16. The method according to claim 1, wherein the binding moieties are bound to a solid support or supports.

17. The method according to claim 16, wherein the solid support or supports is a collection of beads or particles.

18. The method according to claim 16, wherein the solid support or supports are selected from the group consisting of beads, fibers, filters, membranes and monoliths.

19. The method according to claim 16, wherein each binding moiety is attached to a different solid support.

20. The method according to claim 16, wherein a plurality of different binding moieties are attached to a single solid support.

21. The method according to claim 1, further comprising, before step (a), the step of culturing a cell that produces the target protein group.

22. The method according to claim 21, wherein the cell is a eukaryotic cell.

23. The method according to claim 21, wherein the cell is a prokaryotic cell.

24. The method according to claim 21, wherein the sample is a cell supernatant.

25. The method according to claim 21, wherein the sample is a cell extract.

26. The method according to claim 1, further comprising, before step (a), the steps of: (i) subjecting a prior sample comprising less than 95% of the target protein group and more than 5% of the contaminating proteins to at least one purification step; and (ii) collecting the sample comprising at least 95% of the target protein group and at most 5% of the contaminating proteins.

27. The method according to claim 1, further comprising the step of: (e) preparing a pharmaceutical composition by combining the collected target protein group with a pharmaceutically acceptable carrier.

28. The method according to claim 1, wherein step (a) is done in a suspension batch process.

29. The method according to claim 1, wherein step (a) is done by passing the sample over a column packed with the library of binding moieties attached to a solid support.

30. The method according to claim 1, wherein step (a) comprises a fluidized bed process.

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