Claims for Patent: 7,666,616
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Summary for Patent: 7,666,616
Title: | Nucleic acid sequences encoding luciferase for expression in filamentous fungi |
Abstract: | The present invention relates to genetic reporters. Specifically, the present invention is directed to a modified gene encoding a luciferase for high level expression in an organism with a bias for cytosine (C) or guanine (G) in the third position of the codon. |
Inventor(s): | Dunlap; Jay C. (Thetford, VT), Loros; Jennifer (Thetford, VT), Mehra; Arun (Hanover, NH), Gooch; Van D. (Morris, MN) |
Assignee: | Trustees of Dartmouth College (Hanover, NH) Regents of the University of Minnesota (Minneapolis, MN) |
Application Number: | 11/576,168 |
Patent Claims: | 1. An isolated nucleic acid molecule comprising the polynucleotide sequence of SEQ ID NO: 1 and encoding a Photinus luciferase of SEQ ID NO:3 for expression in an organism
with a bias for cytosine (C) or guanine (G) in the third position of the codon, wherein the nucleic acid sequence has a G and C content of at least 50% and an intron comprising SEQ ID NO:5.
2. A vector comprising the nucleic acid molecule of claim 1. 3. An isolated host cell containing the vector of claim 2. 4. A method for enhancing the level of a luciferase enzyme in an organism with a bias for C or G in the third position of the codon comprising introducing into an organism with a bias for C or G in the third position of the codon a nucleic acid molecule of claim 1, thereby enhancing the level of luciferase enzyme produced in the organism. 5. A kit for expressing a luciferase enzyme in an organism with a bias for C or G in the third position of the codon comprising the nucleic acid molecule of claim 1. 6. An isolated nucleic acid molecule comprising SEQ ID NO:1. 7. The nucleic acid molecule of claim 6, further comprising an intron of SEQ ID NO:5. |
Details for Patent 7,666,616
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | 06/04/1986 | ⤷ Try a Trial | 2024-09-30 |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | ⤷ Try a Trial | 2024-09-30 | |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | Injection | 103132 | ⤷ Try a Trial | 2024-09-30 | |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
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