Claims for Patent: 7,560,276
✉ Email this page to a colleague
Summary for Patent: 7,560,276
Title: | Soft tissue repair and regeneration using postpartum-derived cells |
Abstract: | Cells derived from postpartum tissue having the potential to support cells of and/or differentiate to cells of a soft tissue lineage, and methods of preparation and use of those postpartum tissue-derived cells, are provided by the invention. The invention also provides methods for the use of such postpartum-derived cells and products related thereto in therapies for conditions of soft tissue. |
Inventor(s): | Harmon; Alexander M. (Clinton, NJ), Harris; Ian Ross (Belle Mead, NJ), Kihm; Anthony J. (Princeton, NJ), Mistry; Sanjay (Bedminster, NJ), Messina; Darin J. (Somerville, NJ), Seyda; Agnieszka (New Brunswick, NJ), Yi; Chin-Feng (Hillsborough, NJ), Gosiewska; Anna (Skillman, NJ) |
Assignee: | Ethicon, Incorporated (Somerville, NJ) |
Application Number: | 10/877,009 |
Patent Claims: | 1. A method of stimulating tissue infiltration in a soft tissue defect in a patient in need thereof, said method comprising (a) contacting mammalian umbilical cord tissue that
is substantially free of blood with a composition comprising dispase and at least one of hyaluronidase and collagenase, thereby yielding isolated cells; (b) optionally expanding said isolated cells in culture; (c) placing said isolated or expanded,
isolated cells onto a scaffold, thereby yielding a scaffold comprising cells; and then (d) administering to said soft tissue defect of said patient said scaffold comprising cells; wherein said isolated or expanded, isolated cells are capable of
self-renewal and differentiation; wherein said isolated or expanded, isolated cells do not express CD117; and wherein said isolated or expanded, isolated cells exhibit increased expression relative to a human cell that is a fibroblast, a mesenchymal
stem cell, or an iliac crest bone marrow cell of an endogenous gene encoding oxidized low density lipoprotein receptor 1, an endogenous gene encoding interleukin 8, and an endogenous gene encoding reticulon 1; and wherein said scaffold comprising cells
stimulates tissue infiltration into said soft tissue defect.
2. The method of claim 1 wherein said isolated cells or said expanded, isolated cells require L-valine for growth and are capable of growth in about 5% to about 20% oxygen. 3. The method of claim 1 wherein said isolated cells or said expanded, isolated cells comprise the following characteristics: potential for at least about 40 doublings in culture; attachment and expansion on a coated or uncoated tissue culture vessel, wherein said coated tissue culture vessel comprises a coating of gelatin, laminin, collagen, polyornithine, vitronectin, or fibronectin; production of vimentin and alpha-smooth muscle actin; and production of CD10, CD13, CD44, CD73, and CD90. 4. The method of claim 1 wherein the isolated cells or the expanded, isolated cells express each of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, and HLA-A,B,C. 5. The method of claim 1 wherein the isolated cells or the expanded, isolated cells do not express any of CD31, CD34, CD45, CD117, CD141, or HLA-DR,DP,DQ, as detected by flow cytometry. 6. The method of claim 1 wherein the tissue comprises blood vessels. 7. The method of claim 1 wherein said step of administering comprises implanting, injecting, or engrafting said scaffold. |
Details for Patent 7,560,276
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Bausch & Lomb Incorporated | VITRASE | hyaluronidase | Injection | 021640 | 05/05/2004 | ⤷ Try a Trial | 2023-06-27 |
Bausch & Lomb Incorporated | VITRASE | hyaluronidase | Injection | 021640 | 12/02/2004 | ⤷ Try a Trial | 2023-06-27 |
Amphastar Pharmaceuticals, Inc. | AMPHADASE | hyaluronidase | Injection | 021665 | 10/26/2004 | ⤷ Try a Trial | 2023-06-27 |
Akorn, Inc. | HYDASE | hyaluronidase | Injection | 021716 | 10/25/2005 | ⤷ Try a Trial | 2023-06-27 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
Make Better Decisions: Try a trial or see plans & pricing
Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.