Claims for Patent: 7,538,259

Summary for Patent: 7,538,259

Title:	Moss expressing promoting regions
Abstract:	Disclosed are isolated nucleic acid molecules encoding wild type nucleus derived moss expression promoting regions (MEPRs) as well as a method for producing recombinant polypeptides using such MEPRs.
Inventor(s):	Rodriguez-Franco; Marta (Freiburg, DE), Jost; Wolfgang (Freiburg, DE), Weise; Andreas (Freiburg, DE), Gorr; Gilbert (Freiburg, DE)
Assignee:	Greenovation Biotech GmbH (Freiburg, DE)
Application Number:	10/568,156
Patent Claims:	1. An isolated nucleic acid molecule comprising a wild-type, nucleus-derived moss expression promoting region (MEPR) comprising SEQ ID NO: 13 or an expression promoting fragment of SEQ ID NO: 13, wherein the isolated nucleic acid molecule comprises a moss promoter. 2. The isolated nucleic acid molecule of claim 1, wherein the MEPR is from Physcomitrella, Funaria, Sphagnum, Ceratodon, Marchantia, or Sphaerocarpos. 3. The isolated nucleic acid of claim 2, wherein the MEPR is from Physcomitrella patens, Funaria hygrometrica and Marchantia polymorpha. 4. The isolated nucleic acid molecule of claim 1, further comprising a 5'-UTR and/or a 5'-intron and/or a 3'UTR. 5. The isolated nucleic acid molecule of claim 1, wherein the MEPR has an expression promoting activity that is at least equal to the expression promoting activity of cauliflower mosaic virus (CaMV) 35S promoter. 6. The isolated nucleic acid molecule of claim 1, wherein the MEPR has an expression promoting activity that is at least 200% of the expression promoting activity of cauliflower mosaic virus (CaMV) 35S promoter. 7. The isolated nucleic acid molecule of claim 6, wherein the MEPR has an expression promoting activity that is at least 500% of the expression promoting activity of cauliflower mosaic virus (CaMV) 35S promoter. 8. The isolated nucleic acid molecule of claim 6, wherein the MEPR has an expression promoting activity that is at least 1000% of the expression promoting activity of cauliflower mosaic virus (CaMV) 35S promoter. 9. The isolated nucleic acid molecule of claim 1, further comprising a coding region for a recombinant polypeptide product under control of the MEPR. 10. The isolated nucleic acid molecule of claim 1, further comprising a selection marker. 11. The isolated nucleic acid molecule of claim 1, further comprising at least one sequence that is homologous to a genomic sequences of a species to be transformed. 12. The isolated nucleic acid molecule of claim 1, further comprising an antisense or ribozyme molecule. 13. A method for the expression of a recombinant polypeptide product in an eukaryotic host cell comprising: providing a recombinant DNA cloning vehicle comprising an isolated nucleic acid molecule encoding an MEPR of claim 12 and a coding sequence for said recombinant polypeptide product under the control of the MEPR; transforming said eukaryotic host cell that does not naturally harbour said coding sequence under the control of said MEPR with the cloning vehicle; culturing the transformed eukaryotic host cell in a suitable culture medium; allowing expression of said recombinant polypeptide; and isolating the expressed recombinant polypeptide. 14. The method of claim 13, wherein said eukaryotic host cell is a plant cell. 15. The method of claim 14, wherein the plant cell is a moss cell. 16. The method of claim 14, wherein the plant cell is a Physcomitrella patens cell. 17. The method of claim 13, wherein said host cell is a protonema moss tissue cell. 18. The method of claim 13, wherein the culture medium is free of added phytohormones. 19. The method of claim 13, wherein the cell is a Physcomitrella, Funaria, Sphagnum, Ceratodon, Marchantia, or Sphaerocarpos cell. 20. The method of claim 13, wherein the host cell expresses said recombinant polypeptide product transiently. 21. The method of claim 13, further defined as a method for industrially producing the polypeptide. 22. The method of claim 13, further defined as a method for providing recombinant cells producing said polypeptide. 23. The method of claim 22, wherein the recombinant cells are further defined as recombinant moss cells expressing said polypeptide. 24. The method of claim 13, further defined as a method for screening and defining consensus sequences for expression promoting regions. 25. The method of claim 13, further defined as a method for recombinant expression of post-translationally modified proteins. 26. The method of claim 25, further defined as a method for production of post-translationally modified proteins. 27. The method of claim 13, further defined as a method for recombinant expression of metabolism modifying proteins.

Details for Patent 7,538,259

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2023-08-11
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2023-08-11
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2023-08-11