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Last Updated: March 29, 2024

Claims for Patent: 7,255,990


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Summary for Patent: 7,255,990
Title:Method for screening genes expressing at desired sites
Abstract: The present invention relates to a method for inferring a plant organ, in which a certain gene is to be expressed, using a part of a base sequence, a method for searching for a gene which is to be expressed at a desired site, and a composition, kit, system and program for carrying out these methods. The present invention also relates to a method for inferring a plant organ, in which a plant gene is to be expressed, based on information about the presence or absence of a base sequence which is highly similar to a transposable element in the vicinity of a protein coding region of a plant gene.
Inventor(s): Higo; Kenichi (Tsukuba, JP), Iwamoto; Masao (Tsukuba, JP)
Assignee: National Institute of Agrobiological Sciences (Ibaraki, JP)
Application Number:10/221,596
Patent Claims:1. A method for detecting a gene which is expressed in a flower and other organs in a rice plant, comprising the steps of: (1) searching a gene population using a Tourist C transposon sequence consisting of SEQ ID NO: 1 as a key sequence, (2) selecting a gene having the transposon sequence in the vicinity of a putative protein coding region, and (3) detecting expression of said gene in the flower and other organs.

2. The method according to claim 1, wherein the expression of said gene includes expression of at least one site selected from a stamen and a pistil.

3. The method according to claim 1, wherein the gene population is a library and the key sequence is a probe sequence.

4. The method according to claim 3, wherein the database is a DNA library.

5. The method according to claim 3, wherein the search is carried out by a search method selected from the group consisting of stringent hybridization, microarray assay, POR, and in situ hybridization.

6. The method according to claim 1, wherein the vicinity of the putative protein coding region is within about 2 kbp downstream of a translation termination codon, and within an intron.

7. A method for inferring an organ of a rice plant containing a flower and other organs in which a gene is expressed, comprising the step of: (1) obtaining information about whether or not a base sequence of a Tourist C transposable element sequence consisting of SEQ ID NO: 1 is present in the vicinity of the gene and when the sequence is present in the vicinity of the gene, inferring that the gene is expressed in the plant organ containing a flower relating to the Tourist C transposable element sequence.

8. The method according to claim 7, wherein the organ containing a flower contains a site selected from the group consisting of a stamen and a pistil.

9. A method for modifying an expression pattern of a gene of a plant, comprising the step of utilizing the sequence of a gene obtained by a method according to claim 7.

Details for Patent 7,255,990

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2039-02-26
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2039-02-26
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2039-02-26
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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