Claims for Patent: 7,244,591

Summary for Patent: 7,244,591

Title:	Yeast transformant producing recombinant human parathyroid hormone and method for producing the hormone
Abstract:	Novel Saccharomyces Cerevisiae mutant strains are provided for producing human parathyroid hormone. The novel strains acre genetically disrupted in at least one of the genes encoding the yapsin family of proteases Yapsin 1, Yapsin 2 and Yapsin 3 and harbor a human parathyroid hormone gene in their genomes. Culturing the novel strains results in the secretion of intact hPTH into culture media at high yield.
Inventor(s):	Rhee; Sang Ki (Seoul, KR), Kana; Hyun Ah (Taejeon, KR), Chung; Bong Hyun (Taejeon, KR), Ko; Su Min (Kyunggi-do, KR)
Assignee:	Dong Kook Pharmaceutical Co., Ltd. (Seoul, KR)
Application Number:	10/363,329
Patent Litigation and PTAB cases:	See patent lawsuits and PTAB cases for patent 7,244,591
Patent Claims:	1. A method for producing a recombinant human parathyroid hormone (hPTH) from a Saccharomyces cerevisiae host, comprising the steps of: subjecting the Saccharomyces cerevisiae host to a deletion mutation in the genes encoding Yapsin 1 (YPS1) and Yapsin 3 (YPS3); transforming the Saccharomyces cerevisiae host with an expression vector containing a gene encoding human parathyroid hormone; and culturing the transformed Saccharomyces cerevisiae host in an appropriate medium. 2. The method as set forth in claim 1, wherein the deletion mutation is carried out by disrupting the genes encoding Yapsin 1 and Yapsin 3 by use of a Saccharomyces cerevisiae selection marker. 3. The method as set forth in claim 1, wherein the Saccharomyces cerevisiae host is further disrupted in the Yapsin 2 (YPS2) gene. 4. A Saccharomyces cerevisiae strain prepared by transforming a Saccharomyces cerevisiae yps1.DELTA.yps3.DELTA.mutant with an expression vector anchoring a human parathyroid hormone gene. 5. The Saccharomyces cerevisiae strain as set forth in claim 4, wherein the Saccharomyces cerevzsiae strain is Saccharomyces cerevisiae SLH16/pG10-hPTH (accession No. KCTC 0815BP) and the expression vector is pG10-hPTH1. 6. A Saccharomyces cerevisiae strain, prepared by transforming a Saccharomyces cerevisiae yps1.DELTA.yps2.DELTA.yps3A mutant with an expression vector anchoring a human parathyroid hormone gene. 7. The Saccharomyces cerevisiae strain as set forth in claim 6, wherein the Saccharomyces cerevisiae strain is Saccharomyces cerevisiae SLH18/pG10-hPTH (accession No. KCTC 0816BP) and the expression vector is pG10-hPTH1.

Details for Patent 7,244,591

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Nps Pharmaceuticals, Inc.	NATPARA	parathyroid hormone	For Injection	125511	01/23/2015		2020-08-31