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Last Updated: March 29, 2024

Claims for Patent: 7,230,166


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Summary for Patent: 7,230,166
Title:Variety of four-leaf clover and a method for breeding the same
Abstract: Disclosed is a new variety of four-leaf clover named \"Lucky Together\", and a method of breeding the same in large quantities. The method of breeding the new variety of four-leaf clover of the present invention comprises the steps of: (a) sterilizing a clover, (b) treating the clover with chemical agent in order to induce mutation and cell division of the clover, (c) roots-inducing of the mutated clover, (d) asexual propagation of the variety of clover, (e) comparing the features of the variety with those of wild-type clover, and (f) identifying a uniformity of the features of the variety. The four-leaf clover according to the present invention has strong nature, strong cold resistance and excellent disease resistance. Furthermore, the clover according to the present invention grows rapidly and well under intensive light, and can compete successfully with weeds. Therefore, the clover can be used not only for feed but also for improving the quality of soil. Thus, the variety of clover has higher commercial and ornamental values.
Inventor(s): Lee; Won-Kyung (Bundang-gu, Seongnam-si, Gyeonggi-do, KR)
Assignee:
Application Number:10/376,759
Patent Claims:1. A method of breeding a new variety of four leaflet clover from a wild-type three-leaflet clover, Trifolium repens L., the method comprising the steps of: (A) providing stem segments containing leaves of the wild-type three leaflet clover, which includes the sub-steps of: (a) cutting each of the leaved stems of the wild-type three-leaflet clover at a length of about 5 cm; (b) removing the leaves from the stem and cutting each of the stems including a growing point at a length of 3-4 cm; (c) transferring the segments into a conical flask and treating them with a solution of ethyl alcohol of 70% for 20 to 30 seconds to sterilize the segments and washing the segments with sterile water three times; (d) treating the segments with a solution of NaOCl of 1.0% for 15 minutes to sterilize the segments and washing the segments with sterile water three times; (B) cutting each of the sterilized segments from step (A)(d) at a length of 2-3 mm in a clean bench, wherein each of the cut segments includes a growing point allowing shoots to grow from the segments; (C) treating the shoots obtained from step (B) with cold water at a temperature of 5.degree. C. for 20 seconds; treating the shoots with Ethyl Methane Sulfonate of 0.1-0.3 .mu.g/L for 3 seconds; washing the shoots with sterilized water; and then incubating the sterilized shoots in a MS basal medium supplemented with 6 Benzylaminopurine of 0.5-2.0 mg/L, Indol-3-Acetic Acid of 0.5 mg/L and .alpha.-Naphthalene Acetic Acid of 0.5 mg/L under 500 lux illumination at 20-22.degree. C.; and (D) obtaining a new variety of four-leaflet clover from the shoots obtained from step (C), including the sub-steps of: (a) transplanting the shoots to soil comprising a 1:1:1 mixture of perlite, peat moss and sterile sand in a greenhouse under the environment of humidity of 90%, temperature of 18-20.degree. C. and illumination intensity of 70%, when a bud and roots are generated in the supplemented MS basal medium; (b) selecting shoots of the four-leaflet clover obtained from step (D)(a) and propagating the shoots asexually by repeated tissue-culture of the segments throughout 5 generations; and (c) subjecting the stems and roots of the four-leaflet clover to dividing/transplanting or cuttage to obtain a new variety of four-leaflet clover.

2. The method as claimed in claim 1, wherein at the step (C) the concentration of 6-Benzylaminopurine is about 1.0-2.0 mg/L and sucrose with a concentration of about 15-20 g/L is further added to the MS basal medium, thereby reducing callus formation and the transparency ratio.

3. A new variety of four-leaflet clover bred according to the method of claim 1 or 2, wherein the new variety of four-leaflet clover has features in comparison to the wild-type three-leaflet clover Trifolium repens L., as disclosed in table 7 below: TABLE-US-00008 TABLE 7 Features New variety of clover Wild-type clover Height 7.about.25 cm 5.about.20 cm Numbers of leaves Four Three Shape of leaves Heart Ellipse Surface of leaf Fluffy Fluffy Periphery of leaf A pattern of teeth of comb A pattern of teeth of comb Length of leaf 0.3.about.2.5 cm 0.7.about.3.0 cm Width of leaf 0.3.about.2.5 cm 0.5.about.2.0 cm Color of leaf Light green Dark green Shape of stem Separated from ground Creeping on ground surface Numbers of stems 6.about.10 stems Irregular Shape of roots Straight Creeping or straight Lifetime Annual Perennial Flowering time June June Shape of flower Raceme having three Raceme receptacles Color of flower White White or pink Heliophilous Less heliophilous heliophilous Disease endurance Strong Strong Cold resistance Strong Strong Growth rate Rapid Rapid ornamental value Valuable (light green, four- Worthless leaflet) (oval shape)

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