Claims for Patent: 7,217,569

Summary for Patent: 7,217,569

Title:	Clonal cultures of primate embryonic stem cells
Abstract:	Disclosed herein are methods for culturing primate embryonic stem cells. These cells are cultured on a prolonged and stable basis in the presence of exogenously supplied fibroblast growth factor and in the absence of animal serum. Preferably there is also a fibroblast feeder layer. Also disclosed is a culture media containing fibroblast feeder layer and the fibroblast growth factor.
Inventor(s):	Thomson; James A (Madison, WI)
Assignee:
Application Number:	10/430,497
Patent Claims:	1. A method of establishing a clonal human embryonic stem cell line capable of sustaining a phenotype of normal embryonic stem cells following prolonged in vitro culture, the method comprising the steps of providing a culture of undifferentiated human embryonic stem cells; removing an individual undifferentiated human embryonic stem cell in a separate culture which includes at least about 4 ng/ml of exogenously supplied human fibroblast growth factor and which is essentially free of mammalian serum; and culturing the individual undifferentiated cell in a serum-free medium which includes at least about 4 ng/ml of exogenously supplied human fibroblast growth factor such that the cell expands into a clonal culture of undifferentiated human embryonic stem cells maintaining the ability to differentiate into endoderm, mesoderm and ectoderm after at least six months of culture. 2. The method of claim 1 wherein the clonal culture of human embryonic stem cells is maintained for over twelve months. 3. The method of claim 1 wherein in the culturing step the serum-free medium contains collagenase. 4. The method of claim 3 wherein the collagenase is collagenase type IV. 5. The method of claim 3 wherein the concentration of collagenase is 1 mg/ml. 6. A method of establishing a clonal human embryonic stem cell line capable of sustaining a phenotype of normal embryonic stem cells following prolonged in vitro culture, the method comprising the steps of providing a culture of undifferentiated human embryonic stem cells; removing an individual undifferentiated human embryonic stem cell from the culture and placing the individual cell in a separate culture which includes at least about 4 ng/ml of basic human fibroblast growth factor and mouse embryonic feeder cells but is essentially free of mammalian serum; and culturing the cell in the separate culture such that the cell expands into a clonal culture of undifferentiated human embryonic stem cells which can maintain the ability to differentiate into endoderm, mesoderm and ectoderm after at least eight months of culture.

Details for Patent 7,217,569

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2020-03-09