Claims for Patent: 7,005,253

Summary for Patent: 7,005,253

Title:	Cold storage solution for organ and biological tissue preservation
Abstract:	Cold storage solutions for the preservation of organs and biological tissues prior to implantation, including a prostaglandin having vasodilatory, membrane stabilizing, platelet aggregation prevention upon reperfusion, and complement activation inhibitory properties, a nitric oxide donor, and a glutathione-forming agent.
Inventor(s):	Polyak; Maximilian (Glenmoore, PA), Arrington; Ben O\'Mar (East Stroudsburg, PA)
Assignee:	Arrington; Ben O\'Mar (East Stroudsburg, PA)
Application Number:	09/976,804
Patent Claims:	1. An organ or biological tissue preservation aqueous cold storage solution comprising: about 1,000 micrograms/L prostaglandin E1, about 10 mg/L nitroglycerin, about 0.2 mg/L N-acetylcysteine, about 50 150 mM potassium lactobionate, about 10 40 mM KH.sub.2PO.sub.4, about 2 8 mM MgSO.sub.4, about 10 50 mM raffinose, about 1 20 mM adenosine, about 1 10 mM allopurinol, and about 40 60 g/L pentastarch. 2. An organ or biological tissue preservation aqueous cold storage solution comprising: about 1,000 micrograms/L prostaglandin E1, about 10 mg/L nitroglycerin, about 0.2 mg/L N-acetylcysteine, about 75 125 mM potassium lactobionate, about 20 30 mM KH.sub.2PO.sub.4, about 3 7 mM MgSO.sub.4, about 20 40 mM raffinose, about 2 10 mM adenosine, about 1 5 mM allopurinol, and about 45 55 g/L pentastarch. 3. An organ or biological tissue preservation aqueous cold storage solution comprising: about 1,000 micrograms/L prostaglandin E1, about 10 mg/L nitroglycerin, about 0.2 mg/L N-acetylcysteine, about 100 mM potassium lactobionate, about 25 mM KH.sub.2PO.sub.4, about 5 mM MgSO.sub.4, about 30 mM raffinose, about 5 mM adenosine, about 1 mM allopurinol, and about 50 g/L pentastarch. 4. The cold storage solution of claims 1, 2, or 3, further comprising NaCl and KOH. 5. The cold storage solution of claim 1, further comprising sterile water. 6. The cold storage solution of claim 5, further comprising about 700 900 mls sterile water. 7. A method for preserving an organ or biological tissue comprising: flushing at least one of a cadaveric organ and tissue with the cold storage solution of claim 1, 2 or 3; allowing the flushed at least one of a cadaveric organ and tissue to be enveloped in the cold storage solution; and storing the at least one of a cadaveric organ and tissue in the cold storage solution in at least one of a deep hypothermic condition and physiological condition. 8. The method of claim 7 wherein the flushing comprises: infusing the solution into vasculature of the at least one of a cadaveric organ and tissue; and exsanguinating the at least one of a cadaveric organ and tissue. 9. The method of claim 7 wherein the storing comprises: replacing blood in vasculature of the at least one of a cadaveric organ and tissue with the solution. 10. The method of claim 7 further comprising: replacing the solution with at least blood to return the at least one of a cadaveric organ and tissue to a normothermic condition. 11. The method of claim 7 further comprising: cooling the solution to below 10.degree. C.; inspecting the cooled solution for precipitates; and removing any precipitates by filtration. 12. A method of preparing the organ or biological tissue preservation solution of claim 1, 2 or 3 comprising: providing a solution with sterile water; adding the potassium lactobionate, the potassium phosphate, the raffinose, the adenosine, the allopurinol and the pentastarch to the solution; and mixing the prostaglandin E1, the nitroglycerin and the N-acetylcysteine into the solution. 13. The method of claim 12 further comprising: mixing the solution until all components are dissolved. 14. The method of claim 12 further comprising: infusing the pentastarch under pressure through a dialyzing filter; centrifuging the prostaglandin E1 under hypothermic conditions; and filtering the centrifuged prostaglandin E1.

Details for Patent 7,005,253

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	ZOSTAVAX	zoster vaccine live	For Injection	125123	05/25/2006		2020-10-13