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Last Updated: March 29, 2024

Claims for Patent: 6,984,518


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Summary for Patent: 6,984,518
Title:DNA construct comprising an FGF1B promoter region operably linked to an SV40 large T antigen encoding sequence
Abstract: A transgenic, non-human mammal useful for assessing the effect of candidate chemotherapeutic drugs on the growth of brain tumors in vivo is provided. Incorporated into the genome of the transgenic mammal, which preferably is a rodent, is a transgene which comprises a promoter comprising the nuclear factor binding region of the RR2 cis acting element of a fibroblast growth factor 1B (FGF1B) promoter. Operably linked to the promoter is reporter gene comprising a sequence which encodes the SV40 large T antigen. A transgenic, non-human mammal useful for identifying and isolating FGF1 producing brain cells. Incorporated into the genome of these transgenic animals is a transgene which comprises a promoter comprising the nuclear factor binding region of the RR2 cis acting element of an fibroblast growth factor 1B (FGF1B) promoter. Operably linked to the promoter is reporter gene comprising a sequence which encodes a protein or polypeptide other than an SV40 large T antigen. A method of obtaining neural stem cells from a sample of cells obtained from an animal is also provided. Such method comprises introducing the FGF1B-detector transgene into a sample of cells that have been obtained from the animal, and assaying for expression of the detectable marker in the cells, wherein cells that express the marker are neural stem cells. The cells which express the detectable marker can then be isolated from the population to provide a sub-population of neural stem cells.
Inventor(s): Chiu; Ing-Ming (Dublin, OH)
Assignee:
Application Number:09/990,249
Patent Claims:1. A DNA construct comprising a human FGF1B promoter as set forth in nucleotide 43 to nucleotide 550 of SEQ ID NO: 2 operably linked to a nucleotide sequence encoding the SV40 large T antigen.

2. A DNA construct comprising a human FGF1B promoter as set forth in nucleotide 10 to nucleotide 580 of SEQ ID NO: 2 operably linked to a nucleotide sequence encoding the SV40 large T antigen.

3. The DNA construct of claim 1 wherein the SV40 large T antigen encoding sequence comprises an intron within said sequence.

4. A DNA construct for providing neural stem cells, said construct comprising the mouse FGF1B promoter operably linked to a sequence encoding the SV40 large T antigen, wherein the sequence of the mouse FGF1B promoter is set forth in SEQ ID NO. 3.

5. A DNA construct consisting of a human FGF1B promoter as set forth in nucleotide 43 to nucleotide 550 of SEQ ID NO: 2 operably linked to a nucleotide sequence encoding the SV40 large T antigen.

6. A DNA construct consisting of a human FGF1B promoter as set forth in nucleotide 10 to nucleotide 580 of SEQ ID NO: 2 operably linked to a nucleotide sequence encoding the SV40 large T antigen.

Details for Patent 6,984,518

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 06/04/1986 ⤷  Try a Trial 2020-11-22
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try a Trial 2020-11-22
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b Injection 103132 ⤷  Try a Trial 2020-11-22
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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