Claims for Patent: 6,787,687

Summary for Patent: 6,787,687

Title:	Rin gene compositions and methods for use thereof
Abstract:	The current invention provides nucleic acid sequences encoding the RIN and MC genes. Compositions comprising these sequences are described, as are plants transformed with such compositions. Further provided are methods for the expression of the RIN and MC genes. The methods of the invention include the direct creation of transgenic plants with the RIN and MC genes by genetic transformnation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
Inventor(s):	Giovannoni; James (Ithaca, NY), Tanksley; Steven (Ithaca, NY), Padmanabhan; Veeraragavan (Ankeny, IA), Ruezinsky; Diane (Woodland, CA), Vrebalov; Julie (Ithaca, NY), White; Ruth (Lansing, NY)
Assignee:
Application Number:	09/614,981
Patent Claims:	1. An isolated nucleic acid sequence comprising a RIN coding sequence, wherein said RIN coding sequence encodes the polypeptide sequence encoded by SEQ ID NO:6. 2. The isolated nucleic acid sequence of claim 1, further defined as comprising from about 17 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 3. The isolated nucleic acid sequence of claim 2, further defined as comprising from about 25 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 4. The isolated nucleic acid sequence of claim 3, further defined as comprising from about 30 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 5. The isolated nucleic acid sequence of claim 4, further defined as comprising from about 40 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 6. The isolated nucleic acid sequence of claim 5, further defined as comprising from about 60 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 7. The isolated nucleic acid sequence of claim 6, further defined as comprising from about 100 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 8. The isolated nucleic acid sequence of claim 7, further defined as comprising from about 200 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 9. The isolated nucleic acid sequence of claim 8, further defined as comprising from about 400 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 10. The isolated nucleic acid sequence of claim 9, further defined as comprising from about 600 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 11. The isolated nucleic acid sequence of claim 10, further defined as comprising from about 800 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 12. The isolated nucleic acid sequence of claim 11, further defined as comprising from about 1000 to about 1172 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:6. 13. The isolated nucleic acid sequence of claim 12, further defined as comprising the nucleic acid sequence of SEQ ID NO:6. 14. The isolated nucleic acid of claim 1, further comprising an enhancer. 15. The isolated nucleic acid of claim 14, wherein said enhancer comprises an intron. 16. The isolated nucleic acid of claim 1, comprising a transcriptional terminator. 17. An expression vector comprising a RIN coding sequence, wherein said RIN coding sequence encodes the polypeptide sequence encoded by SEQ ID NO:6. 18. The expression vector of claim 17, wherein said RIN coding sequence is operably linked to a heterologous promoter. 19. The expression vector of claim 18, wherein said RIN coding sequence is oriented antisense relative to said heterologous promoter. 20. The expression vector of claim 17, wherein said heterologous promoter is selected from the group consisting of CaMV 35S, CaMV 19S, nos, Adh, histone, ribulose bisphosphate carboxylase, R-allele, root cell promoter, .alpha.-tubulin, ABA-inducible promoter, turgor-inducible promoter, rbcS, sucrose synthetase 1, alcohol dehydrogenase 1, pea small subunit RUBP carboxylase, Ti plasmid mannopine synthase, Ti plasmid nopaline synthase, petunia chalcone isomerase, bean glycine rich protein 1, CaMV 35s transcript, Potato patatin, actin, cab, PEPCase and S-E9 small subunit RuBP carboxylase promoter. 21. The expression vector of claim 17, further defined as comprising a selectable marker selected from the group consisting of phosphinothricin acetyltransferase, glyphosate resistant EPSPS, aminoglycoside phosphotransferase, hygromycin phosphotransferase, neomycin phosphotransferase, dalapon dehalogenase, bromoxynil resistant nitrilase, anthranilate synthase and glyphosate oxidoreductase. 22. The expression vector of claim 17, further defined as a linear nucleic acid segment. 23. The expression vector of claim 17, further defined as a plasmid vector. 24. The expression vector of claim 17, further comprising an enhancer. 25. The expression vector of claim 24, further comprising a nucleic acid sequence encoding a transit peptide. 26. The expression vector of claim 17, wherein said RIN coding sequence is operably linked to a heterologous terminator. 27. The expression vector of claim 26, wherein said terminator is a nos terminator. 28. The expression vector of claim 17, wherein said RIN coding sequence comprises the nucleic acid sequence of claim 2. 29. The expression vector of claim 17, wherein said RIN coding sequence comprises the nucleic acid sequence of claim 13. 30. A transgenic plant stably transformed with the expression vector of claim 17. 31. The transgenic plant of claim 30, wherein said plant is a tomato plant. 32. The transgenic plant of claim 30, further defined as a fertile R.sub.0 transgenic plant. 33. A seed of the fertile R.sub.0 transgenic plant of claim 32, wherein said seed comprises said expression vector. 34. The transgenic plant of claim 30, further defined as a progeny plant of any generation of a fertile R.sub.0 transgenic plant, wherein said R.sub.0 transgenic plant comprises said expression vector. 35. A seed of the progeny plant of claim 34, wherein said seed comprises said expression vector. 36. A crossed fertile transgenic plant prepared according to the method comprising the steps of: (i) obtaining a fertile transgenic plant comprising a selected DNA comprising a RIN coding sequence, wherein said RIN coding sequence encodes the polypeptide sequence encoded by SEQ ID NO:6; (ii) crossing said fertile transgenic plant with itself or with a second plant lacking said selected DNA to prepare the seed of a crossed fertile transgenic plant, wherein said seed comprises said selected DNA; and (iii) planting said seed to obtain a crossed fertile transgenic plant. 37. A seed of the crossed fertile transgenic plant of claim 36, wherein said seed comprises said selected DNA. 38. The crossed fertile transgenic plant of claim 36, wherein said crossed fertile transgenic plant is a tomato plant. 39. The crossed fertile transgenic plant of claim 36, wherein said second plant is an inbred plant. 40. The crossed fertile transgenic plant of claim 39, further defined as a hybrid plant. 41. A method of manipulating the phenotype of a tomato plant comprising the steps of: (i) obtaining an expression vector comprising a RIN coding sequence in sense or antisense orientation, wherein said RIN coding sequence encodes the polypeptide sequence encoded by SEQ ID NO:6; (ii) transforming a recipient tomato plant cell with said expression vector; and (iii) regenerating a transgenic tomato plant from said recipient plant cell, wherein the phenotype of said plant is altered based on the expression of said RIN coding sequence in sense or antisense orientation. 42. The method of claim 41, wherein said step of transforming comprises a method selected from the group consisting of microprojectile bombardment, PEG mediated transformation of protoplasts, electroporation, silicon carbide fiber mediated transformation, and Agrobacterium-mediated transformation. 43. The method of claim 42, wherein said step of transforming comprises Agrobacterium-mediated transformation. 44. A method of plant breeding comprising the steps of: (i) obtaining a transgenic plant comprising a selected DNA comprising a RIN coding sequence, wherein said RIN coding sequence encodes the polypeptide sequence encoded by SEQ ID NO:6; and (ii) crossing said transgenic plant with itself or a second plant. 45. The method of claim 44, wherein said second plant is an inbred plant. 46. The method of claim 44, further comprising the steps of: (iii) collecting seeds resulting from said crossing; (iv) growing said seeds to produce progeny plants; (v) identifying a progeny plant comprising said selected DNA; and (vi) crossing said progeny plant with itself or a third plant. 47. The method of claim 46, wherein said second plant and said third plant are of the same genotype. 48. The method of claim 46, wherein said second and third plants are inbred plants. 49. A transgenic plant cell stably transformed with a selected DNA comprising a RIN coding sequence, wherein said RIN coding sequence encodes the polypeptide sequence encoded by SEQ ID NO:6. 50. The transgenic plant cell of claim 47, wherein said cell is from a tomato plant. 51. The method of claim 36, wherein said selected DNA comprises the expression vector of claim 17.

Details for Patent 6,787,687

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2019-07-12
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2019-07-12
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2019-07-12