You’re using a public version of DrugPatentWatch with 5 free searches available | Register to unlock more free searches. CREATE FREE ACCOUNT

Last Updated: March 29, 2024

Claims for Patent: 6,566,073


✉ Email this page to a colleague

« Back to Dashboard


Summary for Patent: 6,566,073
Title: Materials and methods involving conditional retention domains
Abstract:Materials and methods involving conditional retention domains (CRDs) are disclosed. Also disclosed are fusion proteins containing CRDs and cells expressing such fusion proteins. In addition, the invention provides novel methods for producing target proteins in vivo using fusion proteins containing conditional retention domains and methods for identifying novel CRDs.
Inventor(s): Rivera; Victor (Arlington, MA), Clackson; Timothy (Cambridge, MA), Rothman; James (New York, NY)
Assignee: Ariad Gene Therapeutics, Inc. (Cambridge, MA)
Application Number:09/420,819
Patent Claims:1. A method for inducing cells to secrete a protein of interest, the method comprising treating a cell containing a fusion protein comprising at least one conditional retention domain ("CRD") and at least one additional domain that is heterologous thereto with a ligand which binds to the CRD, the treating being at a concentration of ligand sufficient to induce secretion of the fusion protein or a portion thereof.

2. A method of claim 1 wherein the fusion protein contains more than one CRD.

3. A method of claim 1 wherein the fusion protein molecules form aggregates with one another in the absence of a ligand which binds to the CRD.

4. A method of claim 1 wherein the CRD is derived from retinol binding protein, FKBP (FK506-binding protein) IgM or alpha1-antitrypsin.

5. A method of claim 1 wherein the CRD comprises an FKBP domain with an amino acid replacement at F36 or W59.

6. A method of claim 5 wherein the CRD comprises an FKBP domain containing the mutation F36M or W59V.

7. A method of claim 1 wherein the heterologous domain of the fusion protein comprises the polypeptide sequence of a protein of interest.

8. A method of claim 7 wherein the protein of interest is a hormone, an endorphin, an antibody or an immunogen.

9. A method of claim 8 wherein the protein of interest is selected from the group consisting of insulin, parathyroid hormone and beta-endorphin.

10. A method of claim 1 wherein the fusion protein comprises an enzymatic cleavage site.

11. A method of claim 10 wherein the cleavage site is a furin cleavage site.

12. A method of claim 11 wherein the furin cleavage site comprises the amino acid sequence SARNRQKR (SEQ ID NO. 1).

13. A method of claim 1 wherein the fusion protein further comprises a secretory signal sequence.

14. A method of claim 13 wherein the secretory signal sequence is the signal sequence from the human growth hormone gene.

15. A method of claim 1 wherein the fusion protein comprises a secretory signal sequence, at least one conditional retention domain, a furin cleavage site, and a polypeptide sequence of interest.

16. A method of claim 15 wherein the fusion protein comprises a secretory signal sequence from human growth hormone, three F36M FKBP domains, a human stromelysin-3 furin cleavage site, and a polypeptide sequence of interest.

17. A method of claim 1 wherein the fusion protein comprises a lysosomal targeting signal.

18. A method of claim 1 wherein the cells are mammalian cells.

19. A method of claim 1 wherein the cells are of human origin.

20. A method of claim 1 wherein the cells are primary cells.

21. A method of claim 1 wherein the cells are in an animal.

Make Better Decisions: Try a trial or see plans & pricing

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.