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Summary for Patent: 6,486,135
|Title:||Nucleic acid respiratory syncytial virus vaccines|
|Abstract:||Non-replicating vectors containing a nucleotide sequence coding for an F protein of respiratory syncytial virus (RSV) and a promoter for such sequence, preferably a cytomegalovirus promoter, are described for in vivo immunization. The nucleotide sequence encloding the RSV F protein may lack a sequence encoding the homologous signal peptide but possessing a heterologous signal peptide enhancing RSV F protein expression. Such non-replicating vectors, including plasmids, also may contain a further nucleotide sequence located adjacent to the RSV F protein encoding sequence to enhance the immunoprotective ability of the RSV F protein when expressed in vivo. Such non-replicating vectors may be used to immunize a host against disease caused by infection with RSV, including a human host, by administration thereto, and may be formulated as immunogenic compositions with pharmaceutically-acceptable carriers for such purpose. Such vectors also may be used to produce antibodies for detection of RSV infection in a sample.|
|Inventor(s):||Li; Xiaomao (Toronto, CA), Ewasyshyn; Mary E. (Thornhill, CA), Sambhara; Suryaprakash (Markham, CA), Klein; Michel H. (Willowdale, CA)|
|Assignee:||Aventis Pasteur Limited (Toronto, CA)|
|Patent Claims:||1. A method of using a gene encoding an RSV F protein lacking the transmembrane region to produce an immune response in a host, which comprises: isolating said gene;
operatively linking said gene to at least one control sequence to produce a plasmid vector, said control sequence directing expression of said RSV F protein when said vector is introduced into a host to produce an immune response to said RSV F protein;
and introducing said vector into the host.
2. The method of claim 1 wherein said at least one control sequence comprises the immediate early cytomegalovirus promoter.
3. The method of claims 1 wherein said gene encoding an RSV F protein encodes an RSV F protein fragment lacking an autologous RSV F signal peptide sequence and includes a sequence encoding a heterologous signal peptide which enhance the level of expression of RSV F protein.
4. The method of claims 3 wherein said gene coding an RSV F protein encoding a signal peptide encodes HSV I gD.
5. The method of claim 4 wherein said at least one control sequence comprises the immediately early cytomegalovirus promoter.
6. The method of claim 5 including the step of: operatively linking said gene to an immunoprotective enhancing sequence which comprises a pair of splice sites to prevent aberrant mRNA splicing to produce an enhanced immunoprotection to said RSV F protein in said host.
7. The method of claim 6 wherein said immunoprotection enhancing sequence is that of rabbit .beta.-globin intron II.
|Applicant||Tradename||Biologic Ingredient||Dosage Form||BLA||Number||Approval Date||Patent No.||Assignee||Estimated Patent Expiration||Status||Orphan||Source|
|Schering||INTRON A||interferon alfa-2b||VIAL||103132||001||1986-06-04||Start Trial||Aventis Pasteur Limited (Toronto, CA)||2015-06-07||RX||search|
|Schering||INTRON A||interferon alfa-2b||VIAL||103132||002||1986-06-04||Start Trial||Aventis Pasteur Limited (Toronto, CA)||2015-06-07||RX||search|
|Schering||INTRON A||interferon alfa-2b||VIAL||103132||003||1986-06-04||Start Trial||Aventis Pasteur Limited (Toronto, CA)||2015-06-07||RX||search|
|>Applicant||>Tradename||>Biologic Ingredient||>Dosage Form||>BLA||>Number||>Approval Date||>Patent No.||>Assignee||>Estimated Patent Expiration||>Status||>Orphan||>Source|
|Country||Patent Number||Estimated Expiration|
|World Intellectual Property Organization (WIPO)||0053767||Start Trial|
|World Intellectual Property Organization (WIPO)||9640945||Start Trial|
|United States of America||5843913||Start Trial|
|>Country||>Patent Number||>Estimated Expiration|
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