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Last Updated: January 29, 2022

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Claims for Patent: 6,483,009

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Summary for Patent: 6,483,009
Title: Antisense intron inhibition of starch branching enzyme expression
Abstract:A method for affecting enzymatic activity in a starch producing organism is described. The method comprises expressing in the organism: (a) a first nucleotide which comprises, partially or completely, a first intron of a gene encoding a class A starch branching enzyme in an antisense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron, (b) together with a second nucleotide sequence which comprises, partially or completely, a second intron of a class B starch branching enzyme in an antisense or sense orientation. Also described are antisense sequences, constructs, vectors, transformed cells, and transgenic organisms.
Inventor(s): Poulsen; Peter (Copenhagen K, DK)
Assignee: Danisco a/s (Copenhagen K, DK)
Application Number:09/367,895
Patent Claims:1. A method for affecting starch branching enzyme activity in a starch producing plant cell, plant tissue, plant organ, or plant, comprising expressing in said plant cell, plant tissue, plant organ, or plant: (a) a first nucleotide sequence which comprises a first intron of a potato gene encoding a class A starch branching enzyme in an antisense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron, (b) linked to a second nucleotide sequence which comprises a second intron of a potato gene encoding a class B starch branching enzyme in an antisense or sense orientation.

2. A method according to claim 1, wherein the activity of at least one of the starch branching enzymes is reduced or eliminated.

3. A method according to claim 1, wherein the first nucleotide sequence comprises the complement of SEQ ID NO:38, or a fragment thereof.

4. A method according to claim 1, wherein the first nucleotide further comprises a promoter.

5. An antisense sequence comprising a nucleotide sequence which is the complement of SEQ ID NO:38, or a nucleotide sequence with at least 95% similarity to SEQ ID NO:38.

6. A vector comprising a first nucleotide sequence which comprises a first intron of a potato gene encoding a class A starch branching enzyme in an antisense orientation, linked to a second nucleotide sequence which comprises a second intron of a potato gene encoding a class B starch branching enzyme in an antisense or a sense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron.

7. A plant cell comprising the vector according to claim 6.

8. A transgenic plant cell, plant tissue, plant organ, or plant, each comprising the vector of claim 6.

9. A transgenic plant comprising the vector of claim 6.

10. The method according to claim 4, wherein thpromoter comprises SEQ ID NO:14.

Details for Patent 6,483,009

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 1986-06-04 ⤷  Try it Free 2017-02-21
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try it Free 2017-02-21
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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