Claims for Patent: 6,483,009

Summary for Patent: 6,483,009

Title:	Antisense intron inhibition of starch branching enzyme expression
Abstract:	A method for affecting enzymatic activity in a starch producing organism is described. The method comprises expressing in the organism: (a) a first nucleotide which comprises, partially or completely, a first intron of a gene encoding a class A starch branching enzyme in an antisense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron, (b) together with a second nucleotide sequence which comprises, partially or completely, a second intron of a class B starch branching enzyme in an antisense or sense orientation. Also described are antisense sequences, constructs, vectors, transformed cells, and transgenic organisms.
Inventor(s):	Poulsen; Peter (Copenhagen K, DK)
Assignee:	Danisco a/s (Copenhagen K, DK)
Application Number:	09/367,895
Patent Claims:	1. A method for affecting starch branching enzyme activity in a starch producing plant cell, plant tissue, plant organ, or plant, comprising expressing in said plant cell, plant tissue, plant organ, or plant: (a) a first nucleotide sequence which comprises a first intron of a potato gene encoding a class A starch branching enzyme in an antisense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron, (b) linked to a second nucleotide sequence which comprises a second intron of a potato gene encoding a class B starch branching enzyme in an antisense or sense orientation. 2. A method according to claim 1, wherein the activity of at least one of the starch branching enzymes is reduced or eliminated. 3. A method according to claim 1, wherein the first nucleotide sequence comprises the complement of SEQ ID NO:38, or a fragment thereof. 4. A method according to claim 1, wherein the first nucleotide further comprises a promoter. 5. An antisense sequence comprising a nucleotide sequence which is the complement of SEQ ID NO:38, or a nucleotide sequence with at least 95% similarity to SEQ ID NO:38. 6. A vector comprising a first nucleotide sequence which comprises a first intron of a potato gene encoding a class A starch branching enzyme in an antisense orientation, linked to a second nucleotide sequence which comprises a second intron of a potato gene encoding a class B starch branching enzyme in an antisense or a sense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron. 7. A plant cell comprising the vector according to claim 6. 8. A transgenic plant cell, plant tissue, plant organ, or plant, each comprising the vector of claim 6. 9. A transgenic plant comprising the vector of claim 6. 10. The method according to claim 4, wherein thpromoter comprises SEQ ID NO:14.

Details for Patent 6,483,009

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2017-02-21
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2017-02-21
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2017-02-21