Claims for Patent: 6,465,250

Summary for Patent: 6,465,250

Title:	Antisense modulation of protein phosphatase 2 catalytic subunit alpha expression
Abstract:	Antisense compounds, compositions and methods are provided for modulating the expression of Protein Phosphatase 2 catalytic subunit alpha. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding Protein Phosphatase 2 catalytic subunit alpha. Methods of using these compounds for modulation of Protein Phosphatase 2 catalytic subunit alpha expression and for treatment of diseases associated with expression of Protein Phosphatase 2 catalytic subunit alpha are provided.
Inventor(s):	Wyatt; Jacqueline (Encinitas, CA)
Assignee:	ISIS Pharmaceuticals, Inc. (Carlsbad, CA)
Application Number:	09/780,049
Patent Claims:	1. A compound 8 to 50 nucleobases in length targeted to nucleobases 10 through 29, nucleobases 55 through 74, or nucleobases 124 through 143 of a 5'-untranslated region, nucleobases 279 through 1099 of a coding region, or nucleobases 1152 through 2140 of a 3'-untranslated region of a nucleic acid molecule encoding human Protein Phosphatase 2 catalytic subunit alpha of SEQ ID NO: 17, an intron region of a nucleic acid molecule encoding human Protein Phosphatase 2 catalytic subunit alpha of SEQ ID NO: 18, nucleobases 111 through 130 of a 5'-untranslated region, nucleobases 265 through 284, nucleobases 291 through 320, nucleobases 475 through 514, nucleobases 550 through 569, nucleobases 607 through 626, nucleobases 662 through 691, nucleobases 768 through 797, nucleobases 863 through 900, nucleobases 925 through 944, nucleobases 970 through 989, nucleobases 998 through 1027, or nucleobases 1066 through 1085 of a coding region, or nucleobases 1138 through 1693 of a 3'-untranslated region of a nucleic acid molecule encoding mouse Protein Phosphatase 2 catalytic subunit alpha of SEQ ID NO: 10, wherein said compound specifically hybridizes with one of said regions and inhibits the expression of one of said human of mouse Protein Phosphatase 2 catalytic subunit alpha nucleic acid molecules. 2. The compound of claim 1 which is an antisense oligonucleotide. 3. A compound up to 50 nucleobases in length comprising at least an 8 nucleobase portion of SEQ ID NO: 20, 21, 22, 24, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 63, 65, 66, 67, 68, 69, 70, 74, 75, 76, 77, 78, 79, 80, 81, 82, 84, 85, 87, 89, 90 or 92 which inhibits the expression of human or mouse Protein Phosphatase 2 catalytic subunit alpha. 4. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified internucleoside linkage. 5. The compound of claim 4 wherein the modified internucleoside linkage is a phosphorothioate linkage. 6. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified sugar moiety. 7. The compound of claim 6 wherein the modified sugar moiety is a 2'-O-methoxyethyl sugar moiety. 8. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified nucleobase. 9. The compound of claim 8 wherein the modified nucleobase is a 5-methylcytosine. 10. The compound of claim 2 wherein the antisense oligonucleotide is a chimeric oligonucleotide. 11. A composition comprising the compound of claim 1 and a pharmaceutically acceptable carrier or diluent. 12. The composition of claim 11 further comprising a colloidal dispersion system. 13. The composition of claim 11 wherein the compound is an antisencse oligonucleotide. 14. A method of inhibiting the expression of human or mouse Protein Phosphatase 2 catalytic subunit alpha in cells or tissues comprising contacting said cells or tissues in vitro with the compound of claim 1 so that expression of human or mouse Protein Phosphatase 2 catalytic subunit alpha is inhibited. 15. The compound of claim 3 which is an antisense oligonucleotide. 16. The compound of claim 15 wherein the antisense oligonucleotide comprises at least one modified internucleoside linkage. 17. The compound of claim 16 wherein the modified internucleoside linkage is a phosphorothioate linkage. 18. compound of claim 15 wherein the antisense oligonucleotide comprises at least one modified sugar moiety. 19. The compound of claim 18 wherein the modified sugar moiety is a 2'-O-methoxyethyl sugar moiety. 20. The compound of claim 15 wherein the antisense oligonuicleotide comprises at least one modified nucleobase. 21. The compound of claim 20 wherein the modified nucleobase is a 5-methylcytosine. 22. The compound of claim 15 wherein the antisense oligonucleotide is a chimeric oligonucleotide. 23. A composition comprising the compound of claim 3 and a pharmaceutically acceptable carrier or diluent. 24. The composition of claim 23 further comprising a colloidal dispersion system. 25. The composition of claim 23 wherein the compound is an antisense oligonucleotide. 26. A method of inhibiting the expression of human or mouse Protein Phosphatase 2 catalytic subunit alpha in cells or tissues comprising contacting said cells or tissues in vitro with the compound of claim 3 so that expression of human or mouse Protein Phosphatase 2 catalytic subunit alpha is inhibited.

Details for Patent 6,465,250

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2039-02-26