Claims for Patent: 6,410,324

Summary for Patent: 6,410,324

Title:	Antisense modulation of tumor necrosis factor receptor 2 expression
Abstract:	Antisense compounds, compositions and methods are provided for modulating the expression of Tumor Necrosis Factor Receptor 2. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding Tumor Necrosis Factor Receptor 2. Methods of using these compounds for modulation of Tumor Necrosis Factor Receptor 2 expression and for treatment of diseases associated with expression of Tumor Necrosis Factor Receptor 2 are provided.
Inventor(s):	Bennett; C. Frank (Carlsbad, CA), Watt; Andrew T. (Vista, CA)
Assignee:	ISIS Pharmaceuticals, Inc. (Carlsbad, CA)
Application Number:	09/844,634
Patent Claims:	1. A compound 8 to 30 nucleobases in length targeted to nucleobases 198 through 1472 of a coding region, or nucleobases 1512 through 3671 of a 3'-untranslated region of a nucleic acid molecule encoding a human Tumor Necrosis Factor Receptor 2 (SEQ ID NO: 3), or nucleobases 4477 through 8921 of an intron region encoding human Tumor Necrosis Factor Receptor 2 (SEQ ID NO: 17), or nucleobases 70-1164 of a coding region or nucleobases 1726-3751 of a 3'-untranslated region of a nucleic acid molecule encoding a mouse Tumor Necrosis Factor Receptor 2 (SEQ ID NO: 10), or nucleobases 698-743 of an intron region of a nucleic acid molecule encoding murine Tumor Necrosis Factor Receptor 2 (SEQ ID NO:98), wherein said compound specifically hybridizes with one or said regions and inhibits the expression of Tumor Necrosis Factor Receptor 2. 2. The compound of claim 1 which is an antisense oligonucleotide. 3. A compound up to 50 nucleobases in length comprising at least an 8-nucleobases portion of SEQ ID NO: 20, 21 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 34, 36, 39, 40, 41, 43, 45, 46, 48, 49, 50, 51, 52, 53, 54, 55, 57, 58, 59, 60, 62, 63, 64, 65, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 117, 118, 119, 120, 121, 122, 123, 126, 127, 128, 129, 132, 133, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 167, 171, 173 or 174 which inhibits the expression of human or mouse Tumor Necrosis Factor Receptor 2. 4. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified internucleoside linkage. 5. The compound of claim 4 wherein the modified internucleoside linkage is a phosphorothioate linkage. 6. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified sugar moiety. 7. The compound of claim 6 wherein the modified sugar moiety is a 2'-O-methoxyethyl sugar moiety. 8. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified nucleobase. 9. The compound of claim 8 wherein the modified nucleobase is a 5-methylcytosine. 10. The compound of claim 2 wherein the antisense oligonucleotide is a chimeric oligonucleotide. 11. A composition comprising the compound of claim 1 and a pharmaceutically acceptable carrier or diluent. 12. The composition of claim 11 further comprising a colloidal dispersion system. 13. The composition of claim 11 wherein the compound is an antisense oligonucleotide. 14. A method of inhibiting the expression of human or mouse Tumor Necrosis Factor Receptor 2 in cells or tissues comprising contacting said cells or tissues in vitro with the compound of claim 1 so that expression of human or mouse Tumor Necrosis Factor Receptor 2 is inhibited. 15. The compound of claim 3 which is an antisense oligonucleotide. 16. The compound of claim 15 wherein the antisense oligonucleotide comprises at least one modified internucleoside linkage. 17. The compound of claim 16 wherein the modified internucleoside linkage is a phosphorothioate linkage. 18. The compound of claim 15 wherein the antisense oligonucleotide comprises at least one modified sugar moiety. 19. The compound of claim 18 wherein the modified sugar moiety is a 2'-O-methoxyethyl sugar moiety. 20. The compound of claim 15 wherein the antisense oligonucleotide comprises at least one modified nucleobase. 21. The compound of claim 20 wherein the modified nucleobase is a 5-methylcytosine. 22. The compound of claim 15 wherein the antisense oligonucleotide is a chimeric oligonucleotide. 23. A composition comprising the compound of claim 3 and a pharmaceutically acceptable carrier or diluent. 24. The composition of claim 23 further comprising a colloidal dispersion system. 25. The composition of claim 23 wherein the compound is an antisense oligonucleotide. 26. A method of inhibiting the expression of human or murine Tumor Necrosis Factor Receptor 2 in cells or tissues comprising contacting said cells or tissues in vitro with the compound of claim 3 so that expression of human or murine Tumor Necrosis Factor Receptor 2 is inhibited.

Details for Patent 6,410,324

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2039-02-26