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Last Updated: April 19, 2024

Claims for Patent: 6,406,897

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Summary for Patent: 6,406,897

Title:	Modified protein, method for preparation thereof and compositions for external application containing the modified protein
Abstract:	A protein modified to improve its stability, said modified protein being coupled to a .beta.-1,3-glucan branched with .beta.-1,6-linkage is provided. A method for the production thereof and a cosmetic or dermatological composition comprising the modified protein are also provided. The modified protein shows an improved stability while retaining its activity and being free of skin irritation.
Inventor(s):	Kim; Mu Sung (Suwon, KR), Lee; Sung Gu (Suwon, KR), Kang; Byung Young (Seoul, KR), Lee; Dong Chul (Sungnam, KR)
Assignee:	Pacific Corporation (Seoul, KR)
Application Number:	09/453,965
Patent Claims:	1. A protein modified to improve its stability, said modified protein being coupled to a .beta.-1,3-glucan branched with .beta.-1,6-linkage, wherein said .beta.-1,3-glucan comprises one .beta.-1,6-linkage for every three .beta.-1,3-main chains. 2. The modified protein of claim 1, wherein said .beta.-1,3-glucan branched with .beta.-1,6-linkage is schizophyllan or scleroglucan. 3. The modified protein of claim 1, wherein said protein is selected from the group consisting of papain, bromelain, ficin, trypsin, chymotrypsin, collagenase, elastase, plasmin, bacterial protease, .alpha.-amylase, glucoamylase, cellulase, pectinase, xylanase, .alpha.-glucosidase, .beta.-glucosidase, .alpha.-galactosidase, .beta.-galactosidase, .beta.-glucanase, chitinase, mannanase, phospholipase, triacylglycerol hydrolase, DNase, RNase, phosphatases, lysozymes, catalases, superoxide dismutases, transglutamninases, peroxidases, photolyases, cytokines, growth factors, hormones, immunoglobulins, lactoferrins, metallothioneins and thioredoxins. 4. A method for modifying protein to improve its stability which comprises the step of coupling the protein to a .beta.-1,3-glucan branched with .beta.-1,6-linkage, wherein said .beta.-1,3-glucan comprises one .beta.-1,6-linkage for every three .beta.-1,3-main chains. 5. A method of claim 4, wherein the coupling comprises the steps of (a) reacting said glucan with periodates to oxidize .beta.-1,6-residues of the glucan to form aldehydes; (b) removing unreacted periodates from the reaction solution; (c) adding to the reaction solution a protein in an amount of 0.00001.about.10.0% by weight and allowing the protein to react with the oxidized glucan; and (d) adding to the reaction solution a reducing agent in an amount of 0.0001.about.1.0% by weight. 6. A method of claim 5, which further comprises the step of (e) washing the product in the step (d). 7. A method of claim 5, wherein, when two or more proteins are modified a first protein is added to the reaction mixture in the step (c) and proteins are added to the reaction mixture in the step (d), wherein not all of the aldehydes formed in step (a) react with the first protein in step (c). 8. A method of claim 4, wherein said protein is selected from the group consisting of papain, bromelain, ficin, trypsin, chymotrypsin, collagenase, elastase, plasmin, bacterial protease, .alpha.-amylase, glucoamylase, cellulase, pectinase, xylanase, .alpha.-glucosidase, .beta.-glucosidase, .alpha.-galactosidase, .beta.-galactosidase, .beta.-glucanase, chitinase, mannanase, phospholipase, triacylglycerol hydrolase, DNase, RNase, phosphatases, lysozymes, catalases, superoxide dismutases, transglutamninases, peroxidases, photolyases, cytokines, growth factors, hormones, immunoglobulins, lactoferrins, metallothioneins and thioredoxins. 9. A method of claim 4, wherein said .beta.-1,3-glucan branched with .beta.-1,6 linkage is schizophyllan or scleroglucan. 10. A method of claim 9 wherein said .beta.-1,3-glucan branched with .beta.-1,6-linkage is one of those subjected to the molecular weight adjustment using a microfluidizer, sonicator or .beta.-1,3-glucanase. 11. A composition for external or topical application which comprises the modified protein of claim 1 in an amount of 0.00001.about.100% by weight based on the total weight of the composition and a dermatologically or cosmetically acceptable vehicle. 12. A composition of claim 11, which is a cosmetic composition selected from the group consisting of cleansing lotion, cleansing cream, skin milk, emollient lotion, massage cream, emollient cream, make-up base, lipstick, facial pack, facial gel, shampoos, rinses, hair-tonics and soaps. 13. A composition of claim 11, which is a dermatological composition selected from the group consisting of lotions, ointments, gels, creams, patches and sprays.

Details for Patent 6,406,897

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965	⤷ Try a Trial	2019-03-19
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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