Claims for Patent: 6,395,959

Summary for Patent: 6,395,959

Title:	Nucleotide sequence encoding the enzyme I SceI and the use thereof
Abstract:	An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
Inventor(s):	Dujon; Bernard (Gif sur Yvette, FR), Choulika; Andre (Paris, FR), Perrin; Arnaud (Paris, FR), Nicolas; Jean-Francois (Noisy le Roi, FR)
Assignee:	Institut Pasteur (Paris, FR) Universite Paris VI/Universite Pierre et Marie Curie (Paris, FR)
Application Number:	08/643,732
Patent Litigation and PTAB cases:	See patent lawsuits and PTAB cases for patent 6,395,959
Patent Claims:	1. A transgenic mouse whose germ cells and somatic cells comprise a nucleotide sequence that comprises an I-SceI restriction site, wherein said nucleotide sequence has been introduced by homologous recombination into the genomic DNA sequence of said transgenic mouse. 2. A method of producing the transgenic mouse of claim 1 comprising the steps of: (a) introducing a nucleotide sequence comprising an I-SceI restriction site into a pronucleus of a fertilized mouse oocyte, wherein said nucleotide sequence is introduced into genomic DNA of said mouse oocyte by homologous recombination; and (b) allowing the fertilized oocyte to develop into a transgenic mouse. 3. The method of claim 2, wherein said I-SceI restriction site is introduced into an exon of a nucleotide sequence coding region. 4. The method of claim 2, wherein said I-SceI restriction site is introduced into an exon, an intron, a promoter region, a locus control region, a pseudogene, a retroelement, a repeated element, a non-functional DNA sequence, a telomere, or a minisatellite region in the genome said mouse oocyte. 5. The method of claim 2, wherein said I-SceI restriction site is introduced with a plasmid comprising a nucleotide sequence comprising the I-SceI restriction site and a nucleotide sequence homologous to a genomic DNA sequence in said mouse oocyte. 6. The transgenic mouse of claim 1, wherein said I-SceI restriction site has been introduced into an exon, an intron, a promoter region, a locus control region, a pseudogene, a retroelement, a repeated element, a non-functional DNA sequence, a telomere, or a minisatellite region. 7. The transgenic mouse of claim 1 wherein the nucleotide sequence comprises an nlsLacZ gene.

Details for Patent 6,395,959

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2012-05-05
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2012-05-05
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2012-05-05