Claims for Patent: 6,372,962
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Summary for Patent: 6,372,962
Title: | Pathogen resistance in plants using CDNA-N/intron constructs |
Abstract: | Constructs comprising (1) a cDNA molecule corresponding to the tobacco N gene and (2) at least a portion of the third intron of the N gene are disclosed. Such constructs are useful for producing TMV resistance in plants. |
Inventor(s): | Baker; Barbara J. (Richmond, CA), Dinesh-Kumar; S. P. (New Haven, CT) |
Assignee: | The Regents of the University of California (Oakland, CA) The United States of America as represented by the Secretary of Agriculture (Washington, DC) |
Application Number: | 09/357,206 |
Patent Claims: | 1. A recombinant nucleic acid molecule comprising cDNA-N/intron 3, wherein said cDNA-N/intron 3 comprises Ngene function and wherein the nucleotide sequence of said cDNA-N/intron 3 has
at least 70% sequence identity to SEQ ID NO: 16, and wherein said cDNA-N/intron 3 encodes an N protein and an N-tr protein that have the activity of SEQ ID NO: 3 and SEQ ID NO: 5, respectively, in conferring tobacco mosaic virus resistance to plants.
2. A nucleic acid according to claim 1 wherein the nucleic acid further comprises pN and 3'-GRS. 3. A nucleic acid molecule according to claim 1 wherein the nucleic acid molecule comprises nucleotides 4282-9534 of the sequence shown in Seq. ID No. 9. 4. A nucleic acid molecule according to claim 2 wherein the nucleic acid molecule comprises the sequence shown in Seq. ID No. 9. 5. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 1. 6. A transgenic plant according to claim 5 wherein the plant is selected from the group consisting of: tobacco, tomato and pepper. 7. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 2. 8. A transgenic plant according to claim 7 wherein the plant is selected from the group consisting of: tobacco, tomato and pepper. 9. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 3. 10. A transgenic plant according to claim 9 wherein the plant is selected from the group consisting of: tobacco, tomato and pepper. 11. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 4. 12. A transgenic plant according to claim 11 wherein the plant is selected from the group consisting of: tobacco, tomato and pepper. 13. A recombinant nucleic acid molecule having a structure and sequence selected from the group consisting of: E1 E2 E3 I3 E4 E5 (SEQ ID NO:16), E1 I1 E2 E3 I3 E4 E5 (SEQ ID NO:17), E1 E2 I2 E3 I3 E4 E5 (SEQ ID NO:18), E1 E2 E3 I3 E4 I4 E5 (SEQ ID NO:19), E1 I1 E2 I2 E3 I3 E4 E5 (SEQ ID NO:20), E1 I1 E2 E3 I3 E4 I4 E5 (SEQ ID NO:21), and E1 E2 I2 E3 I3 E4 I4 E5 (SEQ ID NO:22) wherein E1-E5 are exons 1-5 of an N gene, and I1-I4 are introns 1-4 of an N gene. 14. A nucleic acid molecule according to claim 13, further comprising pN operably linked to E1 and 3'-GRS operably linked to E5. 15. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 14. 16. A transgenic plant according to claim 15, wherein the plant is selected from the group consisting of: tobacco, tomato and pepper. 17. A recombinant nucleic acid molecule comprising a promoter sequence, an open reading frame, an intron and a 3' regulatory sequence of an N gene, wherein: (a) the open reading frame is a cDNA-N sequence having 5' and 3' ends; (b) the intron is a biologically active intron 3 of the N gene; (c) the promoter sequence is a biologically active pN of the N gene; (d) the 3' regulatory region is a biologically active 3'-GRS of the N gene; and wherein: the promoter sequence is operably linked to the 5' end of the open reading frame, the intron is positioned within the open reading frame at a position corresponding to the position of the intron in the N gene, the 3' regulatory region is operably linked to the 3' end of the open reading frame, and the recombinant nucleic acid molecule comprises N gene function, and comprises a nucleotide sequence having at least 70% sequence identity to SEQ ID NO: 9 and encodes an N protein and an N-tr protein that have the activity of SEQ ID NO: 3 and SEQ ID NO: 5, respectively, in conferring tobacco mosaic virus resistance to plants. 18. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 17. 19. A transgenic plant according to claim 18, wherein the plant is selected from the group consisting of: tobacco, tomato and pepper. 20. A recombinant nucleic acid molecule comprising N gene function and comprising a nucleic acid sequence having at least 70% sequence identity to a sequence selected from the group consisting of SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, and SEQ ID NO: 22, wherein said recombinant nucleic acid molecule encodes an N protein and an N-tr protein that have the activity of SEQ ID NO: 3 and SEQ ID NO: 5, respectively, in conferring tobacco mosaic virus resistance to plants. 21. A nucleic acid molecule according to claim 20, further comprising an operably linked pN and an operably linked 3'-GRS. 22. A transgenic plant comprising a recombinant nucleic acid molecule according to claim 21. 23. A transgenic plant according to claim 22, wherein the plant is selected from the group consisting of: tobacco, tomato and pepper. |
Details for Patent 6,372,962
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | 06/04/1986 | ⤷ Try a Trial | 2018-07-20 |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | ⤷ Try a Trial | 2018-07-20 | |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | Injection | 103132 | ⤷ Try a Trial | 2018-07-20 | |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
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