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Last Updated: April 23, 2024

Claims for Patent: 6,368,847

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Summary for Patent: 6,368,847

Title:	Selective media for recovery and enumeration of campylobacters
Abstract:	A composition for recovery and enumeration of Campylobacter species that includes a selective Campylobacter medium plus an indicator, 2,3,5-triphenyltetrazolium chloride. Improved selective media including the indicator, contains a nutrient medium with an energy source, agar, a reducing agent, and selective agents. The selective agents are a mixture of agents selected from cycloheximide, cefoperazone, vancomycin, trimethoprim, polymyxin B; rifampicin, amphotericin, cefoperazone, vancomycin, trimethoprim, and nystatin. The salts of these agents are also useful.
Inventor(s):	Line; John Eric (Watkinsville, GA), Garrish; Johnna Kennedy (Hull, GA), Glassmoyer Pearson; Kirsten Elizabeth (Athens, GA)
Assignee:	The United States of America, as represented by the Secretary of Agriculture (Washington, DC)
Application Number:	09/583,529
Patent Claims:	1. A medium for recovery of Campylobacter species comprising: about 43 grams/liter of Brucella agar; about 50 milliliters/liter of lysed horse blood; or a blood-free composition containing: about 1.0 grams/liter .alpha.-ketoglutaric acid, about 0.6 grams/liter sodium carbonate, about 3 grams/liter yeast extract, and about 0.01 grams/liter hemin; about 33 milligrams/liter of cefoperizone or a cefoperizone salt; about 0.5 gram/liter ferrous sulfate; about 0.2 grams/liter of sodium bisulfite; about 0.5 grams/liter of pyruvic acid; about 10 milligrams/liter of vancomycin or a vancomycin salt; about 5 milligrams/liter of trimethoprim or a trimethoprim salt; about 0.35 milligrams/liter of polymyxin B or a polymyxin B salt; about 200 milligrams/liter of 2,3,5-triphenyltetrazolium chloride; and an antibiotic composition containing: (a) about 100 milligrams/liter of cycloheximide or a cycloheximide salt; or (b) about 10 milligrams/liter rifampicin or a rifampicin salt and about 25 milligrams/liter of amphotericin or an amphotericin salt; or (c) about 10 milligrams/liter of rifampicin or a rifampicin salt and about 50 milligrams/liter of nystatin or a nystatin salt. 2. A medium for recovery of Campylobacter species comprising: about 40-50 grams/liter Brucella Agar; about 20-100 milliliters/liter lysed horse blood or a blood-free composition containing: about 0.5-2.5 grams/liter .alpha.-ketoglutaric acid, about 0.4-0.8 grams/liter sodium carbonate, about 2-5 grams/liter yeast extract, and about 5-25 milligrams/liter hemin; about 20-50 milligrams/liter cefoperizine or a cefoperizine salt; about 0.1-1.0 grams/liter ferrous sulfate; about 0.05-5 grams/liter sodium bisulfate; about 0.1-1.0 grams/liter pyruvic acid; about 5-20 milligrams/liter vancomycin or a vacomycin salt; about 2.5-10 milligrams/liter trimethoprim or a trimethoprim salt; about 0.3-0.75 milligrams/liter polymyxin B or a polymyxin B salt; about 100-200 milligrams/liter 2,3,5-triphenyltetrazolium chloride; and an antibiotic composition containing: (a) about 100-400 milligrams/liter cycloheximide or a cycloheximide salt, or (b) about 5-20 milligrams/liter rifampicin or a rifampicin salt and about 10-40 milligrams/liter amphotericin or an amphotericin salt; or (c) about 5-20 milligrams/liter rifampicin or a rifampicin salt and about 20-80 milligrams/liter nystatin or a nystatin salt. 3. A medium for recovery of Campylobacter species comprising: about 43 grams/liter of Brucella agar, about 50 milliliters/liter of lysed horse blood, about 33 milligrams/liter of cefoperizone or a cefoperizone salt, about 0.5 gram/liter ferrous sulfate, about 0.2 grams/liter of sodium bisulfite, about 0.5 grams/liter of pyruvic acid, about 10 milligrams/liter of vancomycin or a vancomycin salt, about 5 milligrams/liter of trimethoprim or a trimethoprim salt, about 0.35 milligrams/liter of polymyxin B or a polymyxin B salt, about 200 milligrams/liter of 2,3,5-triphenyltetrazolium chloride, and about 100 milligrams/liter of cycloheximide or a cycloheximide salt. 4. A medium for recovery of Campylobacter species comprising: about 43 grams/liter of Brucella agar, about 1.0 grams/liter .alpha.-ketoglutaric acid, about 0.6 grams/liter sodium carbonate, about 3 grams/liter yeast extract, about 0.01 grams/liter hemin, about 33 milligrams/liter of cefoperizone or a cefoperizone salt, about 0.5 gram/liter ferrous sulfate, about 0.2 grams/liter of sodium bisulfite, about 0.5 grams/liter of pyruvic acid, about 10 milligrams/liter of vancomycin or a vancomycin salt, about 5 milligrams/liter of trimethoprim or a trimethoprim salt, about 0.35 milligrams/liter of polymyxin B or a polymyxin B salt, about 200 milligrams/liter of 2,3,5-triphenyltetrazolium chloride, and about 100 milligrams/liter of cycloheximide or a cycloheximide salt. 5. A medium for recovery of Campylobacter species comprising: about 43 grams/liter of Brucella agar, about 1.0 grams/liter .alpha.-ketoglutaric acid, about 0.6 grams/liter sodium carbonate, about 3 grams/liter yeast extract, about 0.01 grams/liter hemin, about 33 milligrams/liter of cefoperizone or a cefoperizone salt, about 0.5 gram/liter ferrous sulfate, about 0.2 grams/liter of sodium bisulfite, about 0.5 grams/liter of pyruvic acid, about 10 milligrams/liter of vancomycin or a vancomycin salt, about 5 milligrams/liter of trimethoprim or a trimethoprim salt, about 0.35 milligrams/liter of polymyxin B or a polymyxin B salt, about 200 milligrams/liter of 2,3,5-triphenyltetrazolium chloride, about 10 milligrams/liter rifampicin or a rifampicin salt, and about 25 milligrams/liter amphotericin or an amphotericin salt. 6. A medium for recovery of Campylobacter species comprising: about 43 grams/liter of Brucella agar, about 1.0 grams/liter .alpha.-ketoglutaric acid, about 0.6 grams/liter sodium carbonate, about 3 grams/liter yeast extract, about 0.01 grams/liter hemin, about 33 milligrams/liter of cefoperizone or a cefoperizone salt, about 0.5 gram/liter ferrous sulfate, about 0.2 grams/liter of sodium bisulfite, about 0.5 grams/liter of pyruvic acid, about 10 milligrams/liter of vancomycin or a vancomycin salt, about 5 milligrams/liter of trimethoprim or a trimethoprim salt, about 0.35 milligrams/liter of polymyxin B or a polymyxin B salt, about 200 milligrams/liter of 2,3,5-triphenyltetrazolium chloride, about 10 milligrams/liter rifampicin, and about 50 milligrams/liter nystatin. 7. A medium for recovery of Campylobacter species comprising: about 40-50 grams/liter Brucella Agar, about 20-100 milliliters/liter lysed horse blood, about 20-50 milligrams/liter cefoperizine or a cefoperizine salt, about 0.1-1.0 grams/liter ferrous sulfate, about 0.05-5 grams/liter sodium bisulfate, about 0.1-1.0 grams/liter pyruvic acid, about 5-20 milligrams/liter vancomycin or a vacomycin salt, about 2.5-10 milligrams/liter trimethoprim or a trimethoprim salt, about 0.3-0.75 milligrams/liter polymyxin B or a polymyxin B salt, about 100-200 milligrams/liter 2,3,5-triphenyltetrazolium chloride, and about 100-400 milligrams/liter cycloheximide or a cycloheximide salt. 8. A medium for recovery of Campylobacter species comprising: about 40-50 grams/liter Brucella Agar, about 0.5-2.5 grams/liter .alpha.-ketoglutaric acid, about 0.4-0.8 grams/liter sodium carbonate, about 2-5 grams/liter yeast extract, about 5-25 milligrams/liter hemin, about 20-50 milligrams/liter cefoperizine or a cefoperizine salt, about 0.1-1.0 grams/liter ferrous sulfate, about 0.05-5 grams/liter sodium bisulfate, about 0.1-1.0 grams/liter pyruvic acid, about 5-20 milligrams/liter vancomycin or a vacomycin salt, about 2.5-10 milligrams/liter trimethoprim or a trimethoprim salt, about 0.3-0.75 milligrams/liter polymyxin B or a polymyxin B salt, about 100-200 milligrams/liter 2,3,5-triphenyltetrazolium chloride, about 5-20 milligrams/liter rifampicin or a rifampicin salt, and about 10-40 milligrams/liter amphotericin or an amphotericin salt. 9. A medium for recovery of Campylobacter species comprising: about 40-50 grams/liter Brucella Agar, about 0.5-2.5 grams/liter .alpha.-ketoglutaric acid, about 0.4-0.8 grams/liter sodium carbonate, about 2-5 grams/liter yeast extract, about 5-25 milligrams/liter hemin, about 20-50 milligrams/liter cefoperizine or a cefoperizine salt, about 0.1-1.0 grams/liter ferrous sulfate, about 0.05-5 grams/liter sodium bisulfate, about 0.1-1.0 grams/liter pyruvic acid, about 5-20 milligrams/liter vancomycin or a vacomycin salt, about 2.5-10 milligrams/liter trimethoprim or a trimethoprim salt, about 0.3-0.75 milligrams/liter polymyxin B or a polymyxin B salt, about 100-200 milligrams/liter 2,3,5-triphenyltetrazolium chloride, about 5-20 milligrams/liter rifampicin or a rifampicin salt, and about 20-80 milligrams/liter nystatin or a nystatin salt.

Details for Patent 6,368,847

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	PANHEMATIN	hemin for injection	For Injection	101246	07/20/1983	⤷ Try a Trial	2019-06-03
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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